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Modeling of ischemic stroke in rats. Cell-mediated gene therapy.
Russia (TaMSA-Tatarstan) - Kazan State Medical University, Kazan
Department of operative surgery and topographic anatomy
Bashirov Farid Vagizovich
Markosyan Vage Arshaluisovich
English, Russian, Armenian
Type of Research Project
- Basic science
What is the background of the project?
There are about 4 types of models of ischemic stroke in animals in the world. These are models such as 2-vascular and 4-vascular ligation, as well as the transient model and occlusion of the middle cerebral artery. Each model has its positive and negative sides. However, among the existing experimental models of brain stroke in animals, there is no way to create a reproducible limited-volume brain infarction with characteristic behavioral anomalies for the ischemic brain region. The creation of such a model will allow preclinical trials of new drugs to assess their effectiveness in restoring "ischemic penumbra" and behavioral skills and is extremely relevant for both fundamental and practical medicine. In our Department, we reproduce all types of ischemic stroke models since 2012. In the last 4 years, we have settled on occlusion of the middle cerebral artery. Because the occlusion of the middle cerebral artery model is one of the models that most accurately simulate human ischemic stroke.
What is the aim of the project?
To choose a convenient model for gene therapy of ischemic stroke.
What techniques and methods are used?
Microsurgical tools, microsurgical skills The simulation is carried out using the standard occlusion of the middle cerebral artery method. The first stage of the operation is a longitudinal incision of the skin from the corner of the eye to the outer opening of the auditory canal, made on the left. The temporal bone is released from the adjacent muscle of the same name and with the help of carbide boron No. 2; a trepanation hole is drilled in the temporal bone with a diameter of 4-5 mm. Under an operating microscope, the Dura mater is excised perpendicular to the axis of the middle cerebral artery, whose occlusion is caused by a thermo coagulator. After that, the trepanation hole is covered with the temporal muscle, the skin is sutured with Dacron. At the second stage of surgery, to preserve blood flow in the Willis circle on the right through a vertical incision along the midline of the neck, the common carotid artery is dissected, which is ligated with a silk surgical thread (3.0), the skin wound is sutured with Dacron. All surgical sutures are treated with 2% iodine solution.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
To study the 2-vascular model of ischemic stroke. To study the 4-vascular model of ischemic stroke. To study occlusion of the middle cerebral artery model of ischemic stroke. The development of some surgical procedures, such as rules of asepsis and antisepsis, the implementation of surgical approaches in rat models, a basic knowledge of the anatomy of the laboratory animals. The study of the algorithm of operations. Performing the operation on the rat under the supervision of the teacher and without the supervision of the teacher.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, preliminary readings only. Our article is enough for a General understanding. All procedures are better absorbed in practice.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of the anatomy of the laboratory animals and experience in microsurgery are eligible.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Sokolov; M. E.; Bashirov; F. V.; Markosyan; V. A.; Povysheva; T. V.; Fadeev; F. O.; Izmailov; A. A.; et al. (2018). Triple-Gene Therapy for Stroke: A Proof-of- Concept in Vivo Study in Rats. Front. Pharmacol. 9; 1–15. doi:10.3389/fphar.2018.00111. IF: 3.831
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