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The effects of selected natural compounds and cytostatic drugs on cytoskeleton-mediated cell death in different cancer cell lines.
Poland (IFMSA-Poland) - Nicolaus Copernicus University Ludwik Rydygier Collegium Medicum, Bydgoszcz
Department of Histology and Embryology Nicolaus Copernicus University in Toruń Collegium Medicum in Bydgoszcz 24 Karłowicza St. 85-092 Bydgoszcz Poland
Dr. hab. n. med. Magdalena Izdebska, prof. UMK,
Dr. hab. n. med. Magdalena Izdebska, prof. UMK, mgr Marta Hałas-Wiśniewska, mgr Wioletta Zielińska, mgr Klaudia Mikołajczyk
Type of Research Project
- Basic science
What is the background of the project?
Compounds of natural origin and cytostatic drugs, due to their availability, have been an attractive topic in anticancer therapy. The exact mechanism of their anticancer action is not clear, especially in the contexts of the cytoskeleton-mediated cell death. The major components of cytoskeleton are vimentin, tubulin and actin. They are critical players in many cellular processes, e.g. in motility, shape of cells, organization of intracellular structures and cell division. Moreover, intermediate filaments provide mechanical support for the plasma membrane. Several studies have indicated that the reorganization of cytoskeleton plays an important role in the initiation and execution of apoptosis, which is the type of cell death clinically desired in an anticancer therapy.
What is the aim of the project?
The aim of the study will be to determine effects on natural substances and cytostatic drugs on the main cytoskeletal proteins (vimentin, tubulin, actin) in selected cancer cell lines. Furthermore, there will be evaluated changes in cell viability, induction of cell death, changes of morphology and ultrastructure.
What techniques and methods are used?
1. Cell culture • cell culture of cancer cell line • cell death analysis • cell cycle analysis • Massman's Tetrazole Test (MTT) assay 2. Light and fluorescence microscopy • Mayer’s haematoxylin staining • Fluorescence staining of vimentin, tubulin, actin 3. Transmission electron microscopy 4. Statistical analysis
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
• Cell culture fixation • Fluorescence staining of the main cytoskeleton components • Cell death and viability analysis (MTT assay, flow cytometry) • Chemical ingredients preparation • Probes evaluation using fluorescence or confocal microscope All protocols and materials as well as qualified employee supervision will be provided
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
During the research exchange seminars on cell biology and life science methods will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Desinfection procedures – being able to maintain sterile enviroment during work, ability to use light microscope, ability to recognize basic cell structures in the transmission electron microscopy
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
- 1. Izdebska M; Zielińska W; Hałas-Wiśniewska M; Grzanka A. Involvement of Actin in Autophagy and Autophagy-Dependent Multidrug Resistance in Cancer. Cancers (Basel). 2019; 20;11(8).
- 2. Izdebska M; Zielińska W; Grzanka D; Gagat M. The Role of Actin Dynamics and Actin-Binding Proteins Expression in Epithelial-to-Mesenchymal Transition and Its Association with Cancer Progression and Evaluation of Possible Therapeutic Targets. Biomed Res Int. 2018; 16;2018:4578373. doi: 10.1155/2018/4578373
- 3. Klimaszewska-Wiśniewska A; Hałas-Wiśniewska M; Izdebska M; Gagat M; Grzanka A; Grzanka D Antiproliferative and antimetastatic action of quercetin on A549 non-small cell lung cancer cells through its effect on the cytoskeleton. Acta Histochem. 2017;119(2):99-112. doi: 10.1016/j.acthis.2016.11.003.
- 4. Grzanka D; Gagat M; Izdebska M Actin is required for cellular Heath Acta Histochem. 201;115(8):775-82. doi: 10.1016/j.acthis.2013.04.002
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