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Mitotic exit regulation by separase
Catalonia (AECS) - University of Barcelona (UB)- Hospital de Bellvitge, Barcelona
Bellvitge Biomedical Research Institute (IDIBELL) Avinguda de la Granvia, 199, 08908 Hospitalet de Llobregat, Barcelona
Dr. Ethel Queralt
Dr. Ethel Queralt
English and Spanish
Type of Research Project
- Basic science
What is the background of the project?
How eukaryotes -including humans- inherit their nuclear genome is a fundamental question in biology which has direct clinical implications, as chromosome missegregation is a leading cause of miscarriages and birth defects, and is tightly linked to malignant tumour progression. Separase is a master mitotic regulator that upon activation will lead to chromosome segregation, Cdc14 activation, spindle elongation and condensation and resolution of the rDNA. The direct separase targets in most of the mitotic events triggered by separase remain unknown. Our major aim is the identification of separase interacting proteins and/or substrates using state-of-the-art genomic and proteomic screenings.
What is the aim of the project?
Study new separase roles during the cell cycle
What techniques and methods are used?
We plan to extend established, state-of-the-art experimental systems using a model organism such as S.cerevisiae to study the regulation of mitosis and chromosome segregation. To address these questions we will use state-of-the-art approaches: CRISPR genome editing, proteomic and genomic screens, as well as biochemical and functional studies. For the 4 weeks project, the student will use yeast genetics in order to validate the candidates previously obtained in genome wide screening.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
For the 4 weeks project, the student will be using yeast genetics in order to validate the candidates for the further study. Those yeast models will be previously obtained in genome wide screening. The student will prepare the yeast strains by deleting or overexpressing specific genes previously obtained in a genome wide screening and will validate the genetic interactions identified with proteomic and genomic screening. The first few days, the students will learn how to do the experiments. Later, the students will perform the experiments and analyse the results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will assist to the lab meeting of the group and if adequate will be participate on them.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
General knowledge in molecular and cellular biology. Cell Cycle knowledge will be desirable. | Subject passed: Molecular and Cellular Biology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Students in biomedical fields
- Barbara Baro; Ethel Queralt; Fernando Monje Casas. Regulation of Mitotic Exit in Saccharomyces cerevisiae. Methods in Molecular Biology: The Mitotic Exit Network. pp. 3 - 17. Springer New York; 2017
- Jativa et al. Cdc14 activation requires coordinated Cdk1-dependent phosphorylation of Net1 and PP2A-Cdc55 at anapahse onset. Cell Mol Life Sci. 2019 Mar 29. Doi: 10.1007/s00018-019-03086-5.
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