Intracellular Calcium Research for Chemoprevention and Neuroprotection
Spain (IFMSA-SPAIN)-University of Valladolid, Valladolid
Institute of Molecular Biology and Genetics Calle Sanz y Forés, 3, 47003 Valladolid
Lucia Nuñez Llorente and Carlos Villalobos Jorge
Lucia Nuñez Llorente and Carlos Villalobos Jorge
English, Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Intracellular calcium is involved in control of different functions as muscle contraction and exocytosis in neurons as well as long-term processes such as cell proliferation, migration and cell death involved in cancer hallmarks, or synaptic plasticity and neuron cell death involved in aging and neurodegenerative disorders. Changes in cell phenotype are associated to changes in intracellular Ca2+ homeostasis, including changes in function and/or expression of molecular players involved in Ca2+ transport and/or signaling. This deep remodeling of intracellular Ca2+ homeostasis is associated to cancer and neuronal aging that contributes to cancer hallmarks, cognitive decline and susceptibility to neurodegenerative disorders such as Alzheimer ́s disease (AD).
What is the aim of the project?
In this project, we plan to address Ca2+ remodeling in colon and brain cancer as well as in neuronal aging. Understanding the remodeling and reverting it will provide new tools for fighting cancer and age-related cognitive decline and neurodegenerative diseases that are among the most important challenges of our society.
What techniques and methods are used?
Firstly, we select primary neurons from tumor samples, and we do a cell culture of those cells to have a bigger population with similar characteristics. some of the main factories in Zaragoza. Secondly, we observe the cycle of the calcium in the cell with intracellular calcium imaging and mithochondrial calcium imaging. Thirdly, we use flash photolysis of caged-IP3 with the objective of remodeling the Calcium address to control the neuronal aging and the formation of cancer. Lastly, using electrophysiological recordings and inmunofluorecence we are allowed to capture the modifications and consequently the results that we will use in the analysis of data.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will first learn the following techniques: - Cell lines, primary neurons, tumor samples - Cell proliferation assay - Intracellular Calcium imaging in live cells - Mitochondrial Ca2+ imaging in live cells - Flash photolysis of caged-IP3 and confocal microscopy - Electrophysiological recordings - Immunofluorescence Is expected that the student will understand those techniques and he will be ready to perform some of them related to the project under the supervision of the tutor. Moreover, we expect an ethusiastic and collaborative attitude.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, there will be some theoretical teaching by the tutors or their co-workers, but no more than 3-4 hours. We usually attend one seminar a week. And the students must read some scientific articles as preliminary readings, no more that 5-6 papers.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic science
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Dental medicine students (IADS members)
- - Amyloid β Oligomers Increase ER-Mitochondria Ca2+ Cross Talk in Young Hippocampal Neurons and Exacerbate Aging-Induced Intracellular Ca2+ Remodeling. Front Cell Neurosci. 2019 Feb 8;13:22.
- - Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells. Cancers. 2019 Jan 13;11(1)