Projects
Name
Evaluation of microbiological and molecular characteristics of isolated Staphylococcus aureus among professionals and students in the health area
University
Universidade Estadual de Londrina (UEL)
Domain
Microbiology
Departement
Department of Pathology
Head
Marcia Regina Eches Perugini
Tutor
Marcia Regina Eches Perugini
Languages
Englush, Portuguese, Spanish
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes No Yes No No Yes Yes No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Staphylococcus aureus has great medical importance because it lives a commensal relationship with the human beings, composing the normal microbiota of the skin and mainly of the nasal mucosa. S. aureus Methicillin-resistant (MRSA) was first reported in 1961, but in the years 90 there was a increase in the number of MRSA infections reported in populations that were not exposed to risk factors of the hospital environment. Such strains, which have been named CA-MRSA (COMMUNITY-ASSOCIATED-MRSA), spread rapidly among the general population and affect patients with and without exposure to the health care environment. On account of the resistance to & #914;-Lactam, the choice for the treatment of infections caused by MRSA is vancomycin, which also has a decrease in sensitivity profiles, being called vancomycin-Intermediate Staphylococcus aureus (VISA), Heteroresistant vancomycin-Intermediate Staphylococcus aureus (Hvisa) and vancomycin-RESISTANT Staphylococcus aureus (VRSA).
What is the aim of the project?
This is an observational, cross sectional and descriptive study, in which the prevalence rate will be measured. Aiming to evaluate the microbiological and molecular characteristics of isolated Staphylococcus aureus from professionals and students from the health area.
What techniques and methods are used?
For this study will be carried out in at least 320 volunteers, which should fit within all the criteria established by this project. Samples will be identified by MANUAL or automated Methodology VITEK2® (BIOMÉRIEUX) and by genotyping, evaluated for antimicrobial susceptibility, determining the minimum inhibitory concentration (MIC) for vancomycin, screened for the identification of Hvisa strains and determined by the population analysis (PAP). It is hoped to determine the prevalence of Staphylococcus aureus in the university community and of health professionals, and that these results can give subsidies to scientifically substantiate the subject, and thus there is a better understanding of how the migration of strains occurs resistant between hospital and community resources
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will take active part in the clinical examination
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will receive instructions and orientations on the everyday activities of the laboratory and the techniques that are used in the project. The student will collect samples, develop the test (molecular biology testing), evaluate the medical records, calculate the severity SCORE and perform a clinical case discussion. In other words, the student will be involved from the sample collection to the processing of the results and their respective discussion, in order to guarantee the student a more practical and complete learning.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. The tutor will indicate books and scientific papers for reading before and during the internship. In addition, the student will also participate in scientific meetings during the time of the internship
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Determination and interest in learning. Subjects passed: Microbiology and antimicrobial infection control
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Dental medicine students (IADS members)
Articles
- Gelatti Luciane Cristina; Bonamigo Renan Rangel; Becker Ana Paula; d'Azevedo Pedro Alves. Methicillin-resistant Staphylococcus aureus: emerging community dissemination. An. Bras. Dermatol. [Internet]. 2009 Oct [cited 2020 Mar 31] ; 84( 5 ): 501-506. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962009000500009&lng=en. https://doi.org/10.1590/S0365-05962009000500009.