Effect of gut microbiome affecting the traits and diseases of human
Korea (KMSA) - Chonbuk National University, Jeonju
Department of microbiology
Seong-Tshool Hong
Seong-Tshool Hong
English, Korean
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
The human gut microbiome is a massive and complex microbial community consisting of 100 trillion microbes in the intestine. The gut microbiome is essential to the health and wellbeing of the host, especially in the case of atherosclerosis, hypertension, obesity, diabetes, metabolic syndrome, inflammatory bowel disease (IBD), gastrointestinal tract malignancies, hepatic encephalopathy, allergies, behavior, intelligence, autism, neurological diseases, and psychological diseases. Our lab is interested in how gut microbiome affects the traits and diseases of human. Recently we developed a microarray-based technology to investigate the function and it effect of gut microbiome. Our methodology would overcome limitation of current metagenome sequencing method which is widely used in gut microbiome researches.
What is the aim of the project?
The project aims to ilucidate the role of gut microbiome on the traits and diseases of human.
What techniques and methods are used?
1. Isolate and maintain intestinal microbes constituting gut microbiome - Making the culture media for intestinal bacteria - Grow intestinal bacteria in anaerobic condition 2. Culture the gut microbiome constituting microbes and break down to fragments - Homogenize bacterial culture by using a bead biter 3. Make microarray by using the microbe fragments 4. Investigate the role of gut microbiome on the traits and diseases of human by the microarray - Hybrizization of microarray with blood - Data analysis
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
In this project, student will investigate the composition of gut microbiota by using a microarray technology which was developed in our lab. 1. Hybridize blood serum with the microarray of gut microbiota 2. Wash the microarray washing buffer 3. Develop the fluorescence signals on the microarray using a fluorescence reader. 4. Compare the fluorescence signals of the microarray from one individual to another. 5. Statistically analyze the fluorescence signals of different individuals by using ImageJ software. 6. Statistically compare the fluorescence signal differenes with the traits of human beings calculated using one-way ANOVA followed by Bonferroni/Tukey multiple tests for individual means using IBM SPSS statistics 21 software.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, Just personal discussion.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
General scientific knowldge (biology, chemistry, medical microbiology, etc)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)