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Role of chimaerins in the regulation of Rac in cancer and glucose metabolism.
Spain (IFMSA-SPAIN)-University of Valladolid, Valladolid
Instituto de Biología y Genética Molecular. c/Sanz y Fores s/n. 47003 Valladolid. Spain.
Type of Research Project
- Basic science
What is the background of the project?
Our laboratory is focused in the study of the signal transduction pathways and biological responses mediated by lipid second messengers through the small family of GTPases. The lipid second messenger diacilglycerol (DAG) is a major regulator in mammalian cells. DAG signaling triggers numerous cellular responses, including cell proliferation, differentiation and apoptosis. DAG actions are mediated by its binding to the C1 domain of various proteins, including regulators of small GTPases of the Rho family. Rho proteins are key regulators of virtually every aspect of cell biology and therefore, their precise regulation is essential for a proper cellular function. Three group of proteins control the activation state of the GTPases: guanosine exchange factors (GEFs), GTPase activating proteins (GAPs) and guanosine dissociation inhibitors (GDIs). Our research focus on the role on biology and physiopathology of the GAP family of proteins named chimaerins.
What is the aim of the project?
Our goal is to elucidate the regulation, signaling pathways and role on biology and physiopathology of these proteins. We focus on the role of these proteins as tumor suppressors in breast and colon cancer as well as it implication in glucose metabolism.
What techniques and methods are used?
Firstly, we selected the animal models to elucidate the regulation and the signalling pathways of the chimareins. Secondly, we use techniques such as western blot, tac activation assays (pull-down), cell culture, transfection, immunofluorescence and confocal analysis to understand the role on biology and physiopathology of these proteins as suppressors in breast and colon cancer and to know their implication in glucose metabolism. Thirdly, we repeat 3 times the study with every technique with the objective of having standard results. Lastly, we analyse and compare the results and the variation to know the role of these chimareins in the targets previously said.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will first learn the following techniques: - Western blot analysis - Cell culture - Transfection of cell lines (with FuGene) - Immunofluorescence - Confocal analysis After being familiar with these techniques, the student will perform a full experiment in which the effect of chimaerins in the cellular process under analysis will be evaluated.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. We will facilitate research papers that will help the student to understand the project. In addition, the will attend the research seminars offered at the IBGM.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Meticulous and detail-oriented, adaptability. Previous experience with lab work
Are there any legal limitations in the student’s involvement
Perform experiments with animal models
Type of students accepted
This project accepts: - Medical students
- The Rac GTPase in Cancer: From Old Concepts to New Paradigms. Kazanietz MG; Caloca MJ. Cancer Res. 2017 Oct 15;77(20):5445-5451.
- Inhibition and termination of physiological responses by GTPase activating proteins. Ligeti E1; Welti S; Scheffzek K. Physiol Rev. 2012 Jan;92(1):237-72.
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