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Identifying new therapeutic targets to boost the anti-tumoral immune response: an analysis of the immune response in brain tumor microenvironment.
Morocco (IFMSA-Morocco), Faculty of Medicine and Pharmacy of Casablanca, Casablanca
Molecular and cellular pathology laboratory, school of medecine and pharmacy, Hassan II university of Casablanca
Pr. Abdallah Badou
Pr. Abdallah Badou
English, French, Arabic
Type of Research Project
- Basic science
What is the background of the project?
The Immune system seems to play a dual role in tumor progression. Indeed, the involvement of the immune system cells both, favorably and adversely, has been confirmed. It appears that it depends on the nature of the immune response generated in each patient. In the case of brain tumors, immune responses may also contribute to induce changes in the patient’s symptoms and behavior, depending on the location of the tumor and the specific types of immune system cells and mediators involved. In this work, we analyze and characterize the immune responses generated within the tumor microenvironment in patients with glioma.
What is the aim of the project?
To study the immune response within the tumor microenvironment to identify new therapeutics targets whose inhibition would boost the anti-tumoral immune response.
What techniques and methods are used?
-RNA (ribonucleic acid) extraction: Total RNA is extracted from the frozen tissue samples using TRIzol reagent (Invitrogen) as described by the manufacturer, cDNA (complementary deoxyribonucleic acid) is synthesized using Tetro Reverse Transcriptase Enzyme. -Real time qPCR (quantitave polymerase chain reaction) assays: Relative quantification of gene expression is analyzed by real-time PCR in the presence of the fluorescent dye SYBR ™ (synergy brands) Green PCR Master Mix. -Flux cytometry: is a technique used to detect and measure characteristics of immune cell populations and gene expression at protein level. -Cell culture: Analysis of the effect of new molecules on immune cell proliferation, or on cancer cell line apoptosis. -Bioinformatic: Analysis of the expression of different genes in other cohorts
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The tasks expected to be accomplished by the student are: -Getting used to laboratory equipment, like laboratory centrifuges, -Understand the basics of immunology, namely the anti-tumor immune response, anti-tumor immunotherapy and the immunomodulatory pathways of cancer … -Analyzing scientific research articles. -Understanding the principle of the techniques used to analyze the samples: learning RNA extraction steps from biopsies and blood, Learning Reverse Transcription steps to generate Cdna from total RNA. Real time polymerase chain reaction (RT-PCR) Learning cells culture steps. Learning flow cytometry. Being able to analyze the results from the performed experiments, using statistics tools like graphpad prism.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, -Preliminary readings of previous studies on immunotherapies in brain cancer cases. -Presentation of research articles.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Have basic informations about immunology ( immune cell populations, innate and adaptive immunity … ) and molecular biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- T lymphocyte subsets in cancer immunity: Friends or foes. Chraa D ; Naim A; Olive D; Badou A. J leukoc Biol. 2019 Feb;105(2):243-255. Doi: 10.1002/JLB.MR0318-097R. Epub2018 Nov 2.Review. https://www.ncbi.nlm.nih.gov/pubmed/30387907
- Kim; Ryul; Bhumsuk Keam; Sehui Kim; Miso Kim; Se Hyun Kim; Jin Wook Kim; Yu Jung Kim; et al. “Differences in Tumor Microenvironments between Primary Lung Tumors and Brain Metastases in Lung Cancer Patients: Therapeutic Implications for Immune Checkpoint Inhibitors.” BMC Cancer 19; no. 1 (January 7; 2019): 19. https://doi.org/10.1186/s12885-018-5214-8. https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-5214-8
- Chuntova P; Downey KM; Hegde B; Almeida ND and Okada H (2019) Genetically Engineered T-Cells for Malignant Glioma: Overcoming the Barriers to Effective Immunotherapy. Front. Immunol. 9:3062. doi: 10.3389/fimmu.2018.03062
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