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(Timisoara) Microbiology diagnosis: identification, resistance phenotypes and study of multi-drug resistance
Romania (FASMR) - University of Medicine and Pharmacy "Victor Babes", Timisoara
Microbiology laboratory, Emergency Clinical County Hospital Timisoara Victor Babes University of Medicine and Pharmacy Timisoara
Prof. Dr. Monica Licker
Prof. Dr. Monica Licker, Anca Goldis
Type of Research Project
- Basic science
What is the background of the project?
Multi-drug resistance is a world-wide problem that seeks an extensive knowledge and multi-disciplinary approach in order to be fully tackled. The laboratory knowledge and skills are vital for any medical professional in order to understand the principles of multi-drug resistance appearance and the methods with which it can be prevented. Our research and practice project has the aim to show the students the main principles of microbiology and to enhance the existing skills and values in order to help the future medical professionals have a better approach in infection treatment and multi-drug resistance prevention. So far we observed that the medical students need assistance in comprehensing the best medical treatment for infections and our project aims to provide a better understanding of this issue by showing the steps of the bacteriological diagnosis and the steps following this point. The outcome of this project is a better understanding of the bacteriological diagnosis and the application of the antibiotic guidelines in the medical practice.
What is the aim of the project?
The aim of our project is to show the students the day-to-day work of the microbiology laboratory and to help them familiarize with the basic diagnosis and identification methods. Further research and special methods will be shown in order to enhance the diagnosis skill and approach of the future medical professionals The study of multi-drug resistance will also be conducted in order to help the students acquire a healthy methodology and approach in the case of hospital acquired infections produced by of multi-drug resistant pathogens. Also, special clinical cases and resistance phenotypes will be discussed in order to help students to identify the optimal therapeutic options.
What techniques and methods are used?
During this project bacteriological diagnosis and techniques will be used, namely: -GRAM stain of the gathered samples -Antibiogram, anti-fungigram interpretation - in order to determine a specific antibiotic suitable for treatment -Culture inoculation - on culture media -Macroscopic examination of the culture media and microscopic examination of the GRAM stained samples -The main steps of the microbiology diagnosis – an internal algorithm used in the lab -Resistant phenotypes interpretation – on the antibiograms and antifungigrams -Principles of sepsis prevention and treatment – internal procedures for achieving effective prevention&treatment -Principles of microbe culture isolation – internal procedures used in the lab for achieving microbial isolation -General microbiology theoretical knowledge Discussion and examination of the evolution of multi-resistant bacteria strains Developing treatments and case simulations for a better comprehension of the acquired knowledge
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The students are expected to use their basic laboratory theoretical and practical skills. Thus, they will have to interpret some antibiograms and antifungigrams. What is more, looking at the culture media, the student will have to recognize the basic pathogens at a macroscopic examination. Besides this, the students are expected to calibrate a microscope by themselves and to prepare a GRAM stain for microbes and fungi and to interpret the pathogen class using a microscopic exam. Having all the information collected so far, one is expected to carry out a bacteriological diagnosis at the end of the analysis and to provide treatment options for the studied pathologies and particular strains. To sum up the month, a presentation will be held in front of the tutors at the end of the project (the subject will be discussed with the tutor) in which they will present the acquired knowledge and skills.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, we will provide theoretical material and teaching via e-mail and on site.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Microbiology, Bacteriology Laboratory theoretical and practical skills: interpret antibiogram, antifungigram, recognize basic pathogens at macroscopic scale, calibrate microscope, interpretation both macro- and microscope.
Are there any legal limitations in the student’s involvement
Microbiology labor protection form (provided on arrival)
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- M (Licker; Monica); Sorescu; T (Sorescu; Teodora); Rus; M (Rus; Maria); Cirlea; N (Cirlea; Natalia); Horhat; F (Horhat; Florin); Jurescu; C (Jurescu; Cristiana); Botoca; M (Botoca; Mircea); Cumpanas; A (Cumpanas; Alin); Timar; R (Timar; Romulus); Muntean; D (Muntean; Delia). Extensively drug-resistant Myroides odoratimimus - a case series of urinary tract infections in immunocompromised patients. INFECTION AND DRUG RESISTANCE; 2018; 11: 743-749; Impact factor 3.443 DOI: 10.2147/IDR.S161069
- Chita; T (Chita; Teodora); Timar; B (Timar; Bogdan); Muntean; D (Muntean; Delia); Baditoiu; L (Baditoiu; Luminita); Horhat; F(Horhat; Florin); Hogea; E (Hogea; Elena); Moldovan; R (Moldovan; Roxana); Timar; R (Timar; Romulus); Licker; M (Licker; Monica). Urinary tract infections in Romanian patients with diabetes: prevalence; etiology; and risk factors. THERAPEUTICS AND CLINICAL RISK MANAGEMENT; 2017; 13: 1-7; Impact factor 1.995 DOI: 10.2147/TCRM.S123226
- D (Muntean; Delia); Licker; M (Licker; Monica); Horhat; F (Horhat; Florin); Dumitrascu; V (Dumitrascu; Victor); Sandesc; D(Sandesc; Dorel); Bedreag; O (Bedreag; Ovidiu); Dugaesescu; D (Dugaesescu; Dorina); Cosnita; DA (Cosnita; Dan A.); Krasta; A (Krasta; Anca); Baditoiu; L (Baditoiu; Luminita). Extensively drug-resistant Acinetobacter baumannii and Proteeae association in a Romanian intensive care unit: risk factors for acquisition. INFECTION AND DRUG RESISTANCE; 2018; 11: 2187-2197; Impact factor 3.443 DOI: 10.2147/IDR.S171288
- Muntean; D (Muntean; Delia); Horhat; FG (Horhat; Florin-George); Baditoiu; L (Baditoiu; Luminita); Dumitrascu; V (Dumitrascu; Victor); Bagiu; IC (Bagiu; Iulia-Cristina); Horhat; DI (Horhat; Delia-Ioana); Cosnita; DA (Cosnita; Dan A.); Krasta; A (Krasta; Anca); Dugaesescu; D (Dugaesescu; Dorina); Licker; M (Licker; Monica). Multidrug-Resistant Gram-Negative Bacilli: A Retrospective Study of Trends in a Tertiary Healthcare Unit. MEDICINA-LITHUANIA; 2018; 54(6). Article Number UNSP 92; Impact factor 1.429 DOI: 10.3390/medicina54060092
- Goldiș Anca; Iasmina Maria Hîncu; Laurențiu Nicolae Ilinca. Multi-resistant bacteria associated with periferic chronic ischemia-isolated within vascular surgery department. The XXth Edition of Craiova International Medical Students Conference 2018 -Anca Goldiș; Diana Carina Iovanovici. Distribution of multi-drug resistant organisms and their impact on the evolution of patients-hospitalized in the intensive care unit. The 22 th International Congress for Students and Young Doctors ″Medis″; Timișoara; 14-17 March 2019
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