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The benefits of Olive oil: Molecular mechanisms of squalene action
Spain (IFMSA-SPAIN)-University of Zaragoza, Zaragoza
Biochemistry and Molecular Biology, Miguel Servet, 177, E- 50013 Zaragoza, Spain
Dr Jesús Osada
Dr Jesús Osada
Type of Research Project
- Basic science
What is the background of the project?
Squalene is a triterpenic compound found in a large number of plants and other sources with a long tradition of research since it was first reported in 1926. Squalene is the main unsaponifiable of virgin olive oil and is quite stable. It has antioxidant, anti‐inflammatory and anti‐atherosclerotic properties in vivo and in vitro. For these reasons, it has been proposed as responsible of the beneficial actions of this oil. We are interested in whether or not this compound is able to exert its action by itself or inside the olive oil.
What is the aim of the project?
To explore molecular mechanisms of action of this compound in vivo and in vitro.
What techniques and methods are used?
First, cell culture to explore gene expressions influenced by the incubation with the compound. Second, in vivo intervention in Apoe-deficient mice with the compound at several doses and vehicles. Third, sampling of liver and intestine and analyses of plasma parameters. Four, gas-chromatography-mass spectrometry to prove that the compound is entering into the organism Five, analyses of gene expression at the mRNA (Reverse transcription polymerase chain reaction) and protein levels (Western blotting) in liver of control and treated mice. Six, statistical analyses of data
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Spectrophotometric determinations of cholesterol, triglycerides are our starting point to verify changes in lipid metabolism and to set up initial groups of mice with identical initial cholesterol. Preparing RNA and transform into cDNA. Analysis by end-point PCR and by quantitative PCR. Homogenizing tissues to prepare Western blotting They will analyze samples form control and treated animals and will analyze the results to verify their statistical significance using Graphpad and SPSS for association studies
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary teaching will be provided by staff along with assays that can verify the skill as already mentioned.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Minimal laboratory training: pipetting, ability to follow a simple protocol of cholesterol assay.
Are there any legal limitations in the student’s involvement
Animal handling can not be done without supervision
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- CURRENT INSIGHTS INTO THE BIOLOGICAL ACTION OF SQUALENE. Mol Nutr Food Res. 62:1800136 2018. doi: 10.1002/mnfr.201800136
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