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Effect of jaborandi essential oil on ovarian reserve
Brazil (IFMSA-Brazil) - Universidade Federal do Piauí - Campus Parnaíba (UFPI - Parnaíba), Parnaíba
Coordenação do Curso de Medicina, Universidade Federal do Piauí - UFPI, Campus Ministro Reis Velloso
Luciana Rocha Faustino (PhD)
Luciana Rocha Faustino (PhD)
English, Spanish, Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Chemotherapeutic agents are often associated with follicular death, leading to an extensive loss of ovarian reserve. As a result, women have early menopause and increased fertility rate. Thus, gonadotoxicity of chemotherapy is an important issue for women in reproductive age since it can deplete their reproductive potential. Therefore, there is a need for a more specific chemotherapeutic agents which do not harm non- neoplastic cells, including germ cells. In this regard, the development of new drugs has been increasing interest in natural substances with anti-tumor activity. Some plants have been highlighted for its high cytotoxic activity, including activity against breast cancer cells, and specificity. However, to our knowledge, there are few studies of the effect of natural chemotherapic agents on ovarian reserve, and hence on female fertility.
What is the aim of the project?
The aim of this project is to study the effect of the oil or extract of some plants leaves on the preservation of ovarian reserve, using animal models.
What techniques and methods are used?
Histological examination: After collection, ovaries will be fixed in paraformaldehyde in phosphate-buffered saline (PBS; pH 7.2) and subsequently dehydrated and embedded in paraffin wax. Tissues will be cut in 8-um sections, mounted on glass slides and stained with periodic acid-Schiff (PAS)–haematoxylin for assessing follicular morphology, follicular counting and to determine follicular number and stroma density using a microscope. Imunohistochemistry technique: After antigen retrieval of fixed ovaries, tissue sections will be incubated with antibodies to analyse proteins related to follicular death (yH2AX and caspase-3) using a microscope. Molecular biology: The presence of messenger ribonucleic acid (mRNA) will be assessed by conventional Reverse transcription polymerase chain reaction (RT-PCR) using primers for cell death, after isolation of total ribonucleic acid (RNA). Statiscal Analysis: Data normality and the homogeneity of variances will be assessed, respectively, via Shapiro-Wilk and Bartlett’s tests. Data will be compared among groups by a one-way ANOVA followed by a Tukey–Kramer Honestly Significant Difference (HSD). All tests will be performed in R 3.5.6 (R Core Team) and differences among groups will be considered significant when P < 0.05. Results will be expressed as the mean ± SEM
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- To prepare chemical reagents for histology, immunohistochemistry, and PCR application; - To learn histological procedures as fixation, tissue processing (dehydration, clearing and infiltration), and staining; - To cut tissue sections to mount microscope slides. - To analyze slides for follicular morphology, follicular counting and to determine follicular number and stroma density using a microscope. - To learn immunohistochemistry procedures as fixation, tissue processing, antigen retrieval and incubation with antibodies addition; - To analyze the presence of proteins related to follicular death (yH2AX and caspase-3) under a microscope. - To learn molecular techniques as RNA extraction and PCR; - To manipulate lab animals (mice) for drugs administration; - To compare results after statistical analysis and to make graphics from the analyzed data. - To collaborate with students involved in the design and implementation of the activities proposed; - To give assistance during articles and abstracts preparation.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be theoretical teaching such as preliminary readings and lectures given by the tutor and her graduated students.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Articles reading and abstracts preparation experience. Subjects passed: Cellular and molecular biology. Histology. Genetics.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Guerrero; D. D.; Mbemya; g. t.; Bruno; J. B.; Faustino; L. R.; Figueiredo; J. R.; Rodrigues; A. P. R. In vitro culture systems as an alternative for female reproductive toxicology studies. 2019. Zygote.
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