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Neutrophil extracellular traps involvement in lung pathology
Ukraine (UMSA) - Danylo Halytsky Lviv National Medical University, Lviv
Department of Histology, Cytology and Embryology; Department of Pathological Anatomy and Forensic Medicine
Rostyslav Bilyy, DSc, Professor
Rostyslav Bilyy, DSc, Professor at the Department of Histology, Cytology and Embryology Volodymyr Vovk, M.D., Ph.D., Ass. Prof. at the Department of Pathological Anatomy and Forensic Medicine
Required: English Accepted: Ukrainian
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Nanoparticles occur in the processing of materials (polishing, grinding), printing on laser printers, in soot and many other household situations. Some nanoparticles can penetrate lung or dermal barriers and enter the circulatory and lymphatic systems of humans and animals. Nanoparticles are able to interact with plasma membrane. Every year lots of people suffer from diseases that are caused by soot inhalation. These small dust particles are formed during coal mining, laser printing, firefighting, etc. Neutrophils are the first line of the bodyʼs defense system against threatening agents. In response to exogenous (bacteria, fungi and tiny nanosized dust), as well as endogenous stimuli in the form of particles (urate crystals and oxalates) granulocyte form the neutrophil extracellular traps (NETs). Besides, at some acute inflammatory conditions neutrophils can form NETs in the bloodstream resulting clotting of vessels. Since lung capillaries are very narrow they are often affected during acute inflammatory conditions (in cases of lung failure of Multiple organ dysfunction syndrome).
What is the aim of the project?
The aim of current work is to investigate the NETs formation and resulting inflammatory reaction under the soot accumulation and/or acute inflammation in lung tissue and vessels.
What techniques and methods are used?
1. Collection of human autopsy samples with signs of lung damage with thrombi or dust particles (heavy smokers and professional diseases). The autopsies are done for every patient of Lviv Regional Clinical Hospital but the samples of lung tissue will be taken only from heavy smokers and people who suffered from professional diseases. The tissue specimens will be fixed in 10% formalin and processed. Paraffin sectioning will be done followed by haematoxylin and eosin staining. 2. Evaluation of histological components involvement via conventional histology, using hematoxylin and eosin staining. 3. Evaluation of neutrophil involvement via immune histochemistry and fluorescent microscopy, which includes the application of an array of fluorochromes to identify cells and sub-microscopic cellular components with a high degree of specificity amid non-fluorescing material.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Observation and collection of autopsy material (student will be able to take part in autopsies under the supervision of tutor); Tissue preparation, histological analysis, Hematoxylin and Eosin staining (preparation of histological material and analyzing under the microscope ); Observation and performing of immune histochemistry analysis, performing of fluorescent microscopy; Data analysis (statistics, results interpretation).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings of 60 hours are required. Papers can be sent via e-mail and studied at home country.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Histology, Pathological histology
Are there any legal limitations in the student’s involvement
Ability to work with autopsy material under supervision of certified professional.
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Escaping the traps of your own hunters. Cleavage of extracellular DNA NETs is essential for inhibiting peripheral organ failure. Matthias Gunzer. 2017 December Doi:10.1126/science.aar2428
- Host DNases prevent vascular occlusion by neutrophil extracellular traps. Miguel Jiménez-Alcázar; Chandini Rangaswamy; Rachita Panda; Josephine Bitterling;Yashin J. Simsek;Andy T. Long;Rostyslav Bilyy;Veit Krenn; Christoph Renné; Thomas Renné;Stefan Kluge;Ulf Panzer; Ryushin Mizuta;Hans Georg Mannherz;Daisuke Kitamura;Martin Herrmann;Markus Napirei;Tobias A. Fuchs. 2017 December Doi: 10.1126/science.aam8897
- Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation. Luis E. Muñoza; Rostyslav Bilyy; Mona H. C. Biermanna; Deborah Kienhöfera; Christian Mauerödera; Jonas Hahna;Jan M. Braunera; Daniela Weidnera; Jin Chena; Marina Scharin-Mehlmannd; Christina Jankoe; Ralf P. Friedriche;Dirk Mielenzf; Tetiana Dumychb; Maxim D. Lootsikb; Christine Schauera; Georg Schetta; Markus Hoffmanna;Yi Zhaoa and Martin Herrmann. Proceedings of the National Academy of Sciences; 113(40); E5856–E5865. Doi: 10.1073/pnas.1602230113
- Neutrophil Extracellular Traps Form a Barrier between Necrotic and Viable Areas in Acute Abdominal Inflammation. Rostyslav Bilyy;Volodymyr Fedorov;Volodymyr Vovk; Moritz Leppkes; Tetiana Dumych;Valentyna Chopyak;Georg Schett and Martin Herrmann. Front. Immunol.; 10 October 2016. Doi: 10.3389/fimmu.2016.00424
- Autoimmune; rheumatic; chronic inflammatory diseases: Neutrophil extracellular traps on parade. Malgorzata Justyna Podolska; Aparna Mahajan; Jasmin Knopf; Jonas Hahn;Sebastian Boeltz; Luis Munoz; Rostyslav Bilyy & Martin Herrmann. October 2018 Doi: 10.1080/08916934.2018.1519804
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