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Effect of increased entropy on cellular survival
Brazil (IFMSA-Brazil) - Universidade Anhembi Morumbi, São Paulo
Laboratory of experimental research Dr Almeida Lima Address : University Anhembi Morumbi, 2nd floor, Street. Dr. Almeida Lima, 1134 - Parque da Mooca, São Paulo - SP, 03164-000 Fone: +55 11 400-1192
English, Portuguese and Spanish
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
In 1982, the American biochemist Albert Lehninger argued that "order" is produced in the cells that are growing and the people who make the compensation for the "created their in their surroundings the course of growth and division." "Living organisms preserve their internal order by eliminating free energy, forming nutrients or solar energy, and returning to their environment an equal amount of energy in the form of heat and entropy." Entropy increases with cell growth and development and decreases in the dissipation of cellular products, heat and in the synthesis of complex molecules. The accumulation of entropy- rich metabolites con lead its cell death. For this reason, we want to study a method to determine the cell survival.
What is the aim of the project?
To establish an experimental platform that allows the measurement of the specific effects of variations of entropy and enthalpy on the cellular viability, elucidating the specific metabolic involved in the process and which ones indicate the cellular dying process.
What techniques and methods are used?
The primary technique to be employed is tissue culture of Jurkat cells, a neoplastic T cell line which grows in RPMI medium (Roswell Park Memorial Institute – created the culture cell product) supplemented with 10% fetal calf serum. The establishment of the study platform involves in a first round of analysis to identify cell behaviour in different pH and osmolality settings followed by observation of the cell ability to survive energy deprivation or an entropic challenge. The exact techniques to be employed have not yet been publicized and cannot be disclosed in this document. This process involves flow cytometry analysis of the cells and fine chemical manipulation of the cell culture media which demand intermediate mathematical and chemical skills.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students are expected to: a) Initially observe and acquaint themselves b) Learn the rules, safety and practices of tissue culture and working in a clean environment c) Participate in working meetings and provide valuable insight d) Participate in the setting of experiments e) Once they are considered fit, they shall receive duties like maintaining tissue culture plates or prepare cell culture media with tutor supervision f) They are expected to survey the tissue culture plates daily and, if need be service them g) If solicited they will be asked to perform cell counting in an Neubauer chamber after teaching h) If solicited they will be asked to prepare the cells for experimentation using a centrifuge i) If solicited they will have to research a particular topic, and present it to the group in a form of seminar or group discussion j) If solicited they might be asked to operate precision equipment like a pH meter (measures the pH), a microscope, a morphometry equipment, an osmometer or a flow cytometer, but only after receiving instructions on how to.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Brief period of training with periodical, reading article, but without seminars, most of the time is clinical laboratory practices.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Ability to work in group, knowledge of biology, skills with tissue culture, microscopy, flow cytometry
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Finger E. Thermodynamics as the driving principle behind the immune system. Einstein (São Paulo). July/September 2012;10(3):386-8
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