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(Bucharest) Micronutrition in diabetes in relation to oxidative and carbonyl stress
Romania (FASMR) - University of Medicine and Pharmacy "Carol Davila", Bucharest
Department of Functional Medicine- Biochemistry The University of Medicine and Pharmacy Carol Davila
Prof Irina Stoian, PhD
Prof Irina Stoian, PhD Assoc Prof. Gaman Laura, Chemist Iosif Liviu, PhD
Type of Research Project
- Basic science
What is the background of the project?
The global prevalence of diabetes among adults over 18 years of age has risen from 4.7% in 1980 to 8.5% in 2014. Diabetes and diabetes complications are associated with increased oxidative and carbonyl stress, but the metabolic events responsible for its development are not understood well. It is shown that increased oxidative and carbonyl stress are supposed to be the consequence of dysfunctional mitochondria, impaired antioxidant defense. Metabolic dysregulation can impact micronutrient status overconsuming specific coenzymes. Micromineral status is also known to be affected in diabetes patients. The main interest of this research project is to study antioxidant defense systems in relation to micronutrition status in diabetes patients, taking into consideration that most antioxidant defense systems require coenzymes and metal ions in order to properly function.
What is the aim of the project?
We aim to investigate red blood cells and plasma antioxidant systems in relation to selected micronutrients (hidrosoluble and lipid soluble vitamins and iron, coppper and zinc) in diabetes patients with and without complications and to compare the results with apparently healthy aged matched subjects.
What techniques and methods are used?
- Isolating superoxide dismutase from red blood cells, separating red blood cells of diabetes patients - Centrifugation - Analyzing specific compounds in red blood cells and plasma of diabetes patients, performing enzyme kinetics - UV-VIS(ultraviolet-visible) spectroscopy - Analyze lipid soluble and hydrosoluble vitamins -HPLC(high-pressure liquid chromatography)
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Firstly, the student is expected to understand how to lable the samples and how to preserve them. After that, he will learn how to: - Separate red blood cells from the whole blood - Isolate superoxide dismutase from red blood cells and perform enzyme kinetic - Analyze plasma enzymes kinetics using a microplate reader - Prepare buffers, solutions and calibrators using the analytical balance and a pH-meter - Perform a calibration curve and analyze specific samples using UV-VIS spectroscopy
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
- The students will have an introductory lecture in oxidative stress (2 hours) and an introductory lecture on basic laboratory techniques that are going to be used (2 hours) - The lectures will be given by Prof Irina Stoian
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare an abstract - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Seriosity The ability to work in a team Basic laboratory skills Subjects passed: Biochemistry
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Octavian Savu; Ovidiu Marius Bradescu; Cristian Serafinceanu; Liviu Iosif; Constantin Ionescu Tirgoviste & Irina Stoian (2013) Erythrocyte caspase-3 and antioxidant defense is activated in red blood cells and plasma of type 2 diabetes patients at first clinical onset; Redox Report; 18:2; 56-62; DOI: 10.1179/1351000213Y.0000000040
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