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New Silk Fibroin Nano Particle Based Direct Load Transfer Biosensor Design for the Diagnosis Of Influenza A H5N1 Virus (NEU AIV Biosensor Design)Project
Northern Cyprus (MSANC) - Near East University, Nicosia
Biotechnologies and Informatics
Research Center of Experimental Health Sciences
Prof. Dr. Tamer Şanlıdağ
Prof. Dr. Tamer Şanlıdağ and Assist. Prof. Ayşe Arıkan Sarıoğlu
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The antigen and proteins of the influenza virus can be detected by commercially available diagnostic kits. These diagnostic kits within average of 30 minutes can diagnose influenza virus with a sensitivity ranging from 51% to 87% with 69-99% accuracy. However, the diagnoses with these kits are made in qualitatively manner. Therefore, development of diagnostic kit technologies with high reliability and with fast quantitative results is needed in the field. Biosensors quantify the physiological and biochemical changes of biological components and integrate into results via electronics, electrochemical, optical or acoustic analog transducers. This study aims to detect the hemagglutinin (HA) glycoprotein found on the electrode surface of the H5N1 virus. The difference between the current systems is that, it will be an improved design that is able to give numerical results which will target the immunofunctional properties of the electroactive nanoparticles, α-H5 monoclonal antibody IgG2 and the α-H1 polyclonal antibody. Silk fibroin nanoparticles will be synthesized by encapsulation with polypyrolyl iron (III) oxide, and with gold nanoparticles the reinforced polymer complex will be cross-linked with the TriEtilene glycol dimethacrylate onto the membrane. In this way, we will be able to design a specific and reliable diagnosis for influenza A H5N1 which can give results in a quantitative and rapid manner.
What is the aim of the project?
The aim of this study is to design silk fibroin nano-based point of care AIV H5N1 biosensor which is able to give high specificity, reliable and fast quantitative results for diagnosis of AIV H5N1.
What techniques and methods are used?
Silk fibroin was purified from Bomybyx mori cocoons by using a thermochemical process called degumming. Furthermore, silk fibroin micro/nano particles was extracted by using ionotropic gelation method with three different cross-linking agents will be immobilized. Sodium tripolyphosphate (TPP), citric acid (CA) and sodium oxalate (SO) will be used to find out the optimum parameters of the synthesis. The sonication method will be used for enhancing the stability and homogenization of particle sizes. The electrochemical characterization of SF micro/nano particles will be measured by PalmSens4 Potentiostat (PalmSensBV, Netherlands) The synthesized magnetic nanoparticles will be used in the design of the NEU AIV H5N1 biosensor.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Purification of silk fibroin. Extraction of Silk fibroin micro/nano particles by using ionotropic gelation method. Immobilization of extracted Silk fibroin micro/nano particles by three different cross-linking agents. The sonication method will be performed to enhance the stability and homogenization of particle sizes. The electrochemical characterization of SF micro/nano particles will be measured by PalmSens4 Potentiostat (PalmSensBV, Netherlands). Students will contribute to the synthesis of magnetic nanoparticles that will be used in the design of the NEU AIV H5N1 biosensor. Students will take part on all of the process under the vision of supervisor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Before the practical sessions, necessary preliminary readings will be supplied by the supervisor and students are responsible for reading them before the session. All details required to perform and understand the methods in the practicals will be explained by the supervisor Assist. Prof. Dr. Ayse Arıkan.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students should be able to work under healthy and safety measures of a laboratory. All necessary methods and techniques will be explained and taught by the supervisor. Subjects passed: Influenza virus, molecular techniques Previous experience with: Laboratory work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
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