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Molecular identyfication of Borrelia burgdorferii sensu lato and Anaplasma phagocytophilum in hard ticks
Poland (IFMSA-Poland) - University of Warmia and Mazury in Olsztyn, Olsztyn
Department of Biochemistry
Dr hab. Malgorzata Dmitryjuk PhD
Dr hab. Malgorzata Dmitryjuk PhD
Type of Research Project
- Basic science
What is the background of the project?
Borrelia burgdorferii sensu lato and Anaplasma phagocytophilum are agents of dangerous ticks-borne diseases - borreliosis and anaplasmosis. Tick-borne infections are no longer confined to rural areas, they are documented with increasing frequency in urban settlements across the world. They are known to case diseases in humans as well as in their companion animals, esspecially in dogs. Borreliosis and anaplasmosis in animals and humans are significantly contributed to by hard ticks. The tick of Ixodes ricinus is the most common in Europe as well as in Poland.
What is the aim of the project?
The aim of the study is to evaluate the prevalence of Borrelia byrgdorferii sensu lato and Anaplasma phagocytophilum in ticks collected from dogs in the urban areas of Warmia-Mazury District (North-west Poland)
What techniques and methods are used?
The experiment will use the following techniques: 1. isolation of DNA from ticks using commercial kits; 2. Polymerase Chain Reaction (PCR) to identify the A. phagocytophilum 16SRNA gene; 3. nested PCR to identify the B. burgdorferii fla gene; 4. restriction analysis of the fla gene amplicons for the identification of B. burgdorferii genospecies; 5. visualization of products in agarose gel.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students will be required to learn basic molecular biology techniques. The student will learn at the tutor's side and will independently perform DNA isolation from ticks, PCR and Nested Polymerase Chain Reaction (nPCR), restrictive analysis of positive amplicons, and agarose gel electrophoresis. Student will learn to use devices such as: a thermal cycler, an electrophoresis apparatus and a device for visualizing gels and will interpret the results. The student will be able to become with the principles of the operation of the research laboratory and extend the knowledge about tick-borne diseases based on scientific literature.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be directly taught by the tutor
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge of basic molecular biology techniques (PCR).
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Król N.; Obiegala A.; Pfeffer M.; Lonc E. And Kiewra D. 2016. Detection of selected pathogens in ticks collected from cats and dogs in the Wrocław Agglomeration; South-West Poland. Parasites Vectors 9:351; DOI 10.1186/s13071-016-1632-0
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