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Effects of cocaine and alcohol co-consume in the brain of a rat model
Spain (IFMSA-Spain) - University of Ciudad Real, Ciudad Real
Dpt. Inorganic, Organic and Biochemistry Faculty of Medicine Camino de Moledores, s/n Ciudad Real, Spain 13071
Inmaculada Ballesteros Yáñez
Inmaculada Ballesteros Yáñez, Carlos Alberto Castillo Sarmiento
Type of Research Project
- Basic science
What is the background of the project?
Drug addiction is a complex and chronic disorder characterized by the compulsive search for drugs for consumption and a difficulty in controlling. Today, it is a global health problem, with health, legal, economic and social consequences affecting approximately 16% of the world's population. To date, therapies developed to control addiction have proved unsatisfactory as many aspects of this process are not well understood. However, there is a neurochemical mechanism common to all drugs that produce addiction in humans which is the increase of the neurotransmitter dopamine (DA), which is responsible for activating the physiological systems of reward. Although we know enough information about the effect of psychoactive substances on the Central Nervous System (CNS), the field of the effect of the combination of these substances is practically unexplored.
What is the aim of the project?
The aim of the project is a multidisciplinary study which uses the self administration of alcohol and cocaine in an animal model in order to analyses protein and gene expression in different brain regions
What techniques and methods are used?
Extraction of RNA and protein from mouse brain tissue using TRIzol reagent -Isolation of RNA and protein -Measure of the protein concentration by Electrophoresis and Western Blot of the isolated protein samples -Real Time Quantitative Polimerase Chain Reaction with a cDNA obtained from the isolated RNA (we make cDNA of the RNA because cDNA is more stable). With this technique we can measure the expression of different genes present in the cDNA
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will work with us and in the meantime will learn and practice: -Preparing buffers -Processing of brain tissue to obtain biomolecules for its quantification. -Gaining experience and management of molecular and biochemical techniques. Isolation of proteins. -Cell cultures maintaining and photograph analysis with software ImageJ. -Data analysis and quantification: data will be analyzed with SPSS so it’s also expected that the student will be able to use this program at the end of the exchange.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, it will be contextual lectures and readings providing by tutors.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Addict Biol. 2019 Jan 8. doi: 10.1111/adb.12706. Central nucleus of the amygdala as a common substrate of the incubation of drug and natural reinforcer seeking. Roura-Martínez D; Ucha M; Orihuel J; Ballesteros-Yáñez I; Castillo CA; Marcos A; Ambrosio E; Higuera-Matas A.
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