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Effects and function of hypoxia-inducible factor-1alpha expression in models of age-related macular degeneration
Sweden (IFMSA-Sweden) - Karolinska Institutet, Stockholm
Depatment of Clinical Neurosciences
Type of Research Project
- Basic science
What is the background of the project?
Hypoxia-Inducible Factors (HIF) is a protein complex which plays an important role in the respons of the body to low concentrations of oxygen, (hypoxia) by increasing vascularisation and it is today very studied because of its therapeutic potential. Previous studies have suggested a role for HIF in angiogenesis (formation of new blood vessels), through intricate crosstalk mechanisms. Although angiogenesis is a physiologic mechanism involved in regulation of the circulatory system, in pathologies critical hypoxia can lead to the formation of abnormal blood vessels and associate with disease.
What is the aim of the project?
We aim to elucidate the effects of HIF protein expression, in a cell line relevant for retina-associated neovascular pathologies.
What techniques and methods are used?
Standard cell culture techniques with cellular exposure to normoxic and hypoxic environments, and signal pathway inhibition. Whole-cell protein extracts to get the sample that will be analysed. First-strand amplification and polymerase chain reactions (PCR) techniques to amplify the DNA. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) will be used to separate proteins with different molecular masses, and Western blotting will be used to further analyse the proteins (the proteins will be denatured first and then then visualised with the help of a antibody-washed membrane and gel electrophoresis). Total RNA extraction will also be done.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be conducting the laboratorial experiments, executing cell and molecular methods (the student will take part of the methods mentioned above; Standard cell culture techniques, Whole-cell protein extracts, Western blotting, Total RNA extraction, SDS-PAGE and PCR). A hands-on approach is necessary, and technical expertise is expected to develop during project progression. Supervision (both technical/ experimental and theoretical) will be provided always along the was as the student works with the laboratorial experiments. Scientific documentation (lab journal, notes, and project summary) will be necessary from the student during the project as well.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
References /publications will be provided as needed by tutors.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Previous laboratory experience preferred. Previous basic knowledge of HIF pathway, also strongly recommended.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- André H et. al. IOVS 2015 (doi: 10.1167/iovs.15-16476) Mammadzada P et. al. Acta Ophthalmol 2016 (doi: 10.1111/aos.13119) Takei A et. al. Sci Rep 2017 (doi: 10.1038/srep42546)
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