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Pancreatic organoid-based screening to identify potential pancreatic tumour-associated human microbiome metabolites.
Sweden (IFMSA-Sweden) - Umea Universitet, Umea
Department of Radiation Sciences
Daniel Öhlund, Professor Tufve Nyholm
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Human microbiome consists of billions of microbes. Vast majority of these microbes are non-pathogenic and are present in homeostasis within different body organs. Subtle changes in the internal or external environment can distursbs this homestasis causing a change in microbiome composition known as as dysbiosis. Several cancer types, including pancretic ductal adenocarcinoma (PDAC), manifests a significant dysbiosis of the gut and oral microbiome. However, it is unclear whether dysbiosis is the cause or a consequence of the carcinogenesis. We propose that identification of the microbial species affected by cancer-related dysbiosis and charctersization of their metabolites can greatly enhance our current understanding of the elusive realtionship between cancer and microbiome.
What is the aim of the project?
To evaluate the effect of secreted metabolites, derived from selected pathogenic bacteria, on survival and proliferation of mouse normal pancreatic, pre-cancerous PanIN and pancreatic tumour organoids.
What techniques and methods are used?
Expanding and maintaining GFP(green fluorescent protein)-expressing derivatives of mouse pancreatic organoids. Single-cell-dissociation of mouse pancreatic organoids. Assaying organoid proliferation after treatment with secreted microbial metabolites and subsequent transcriptome analysis of organoids in an assay condition showing optimum effect.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be supervised by a post-doc and will take part in the daily lab work. The student will be assigned a specific task, and during the 4 weeks in the lab the student will be taught the techniques required for this task. Examples of tasks can be to expand organoids and set up cell culture experiments, extract RNA from tissue or cultured cells and perform quantitative polymers chain reaction (PCR).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be sent list of literature before starting in the lab. This will give a background to the research field.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Boj S et al. 2015 Organoid models of human and mouse ductal pancreatic cancer. Cell 160: 324-38.
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