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Identification of new generation biomarkers for diagnostic evaluation of patients with systemic lupus erythematosus
Bulgaria (AMSB) - Medical University of Varna, Varna
Department of Biochemistry, Molecular Medicine and Nutrigenomics
Assos. Prof. Yoana Kiselova
Assoc. Prof. Maria Atanasova Radanova, PhD
Type of Research Project
- Basic science
What is the background of the project?
Lupus erythematosus is a very diverse condition. Its autoimmune pathogenesis is responsible for the variety of systematic expression and its different severity. The onset of this condition is at the young age, frequently before 40 years of age. In Bulgaria it is not easily recognized due to the diversity of the symptoms. Many patients go to different specialists and give a lot of money for tests that prove to be inconclusive. This delays the diagnosis and the possible treatment which can aggravate the condition. In Bulgaria there has not been many researches on this topic. The project is designed in order to simplify the diagnosing of lupus in early stages which will help with the treatment of the condition.
What is the aim of the project?
The aim of the project is to identify new generation biomarkers which will help in diagnosing the activity of systemic lupus erythematosus and will help in the observation of the patient.
What techniques and methods are used?
Patients with diagnosed systemic lupus erythematosus will be selected. They will be thoroughly interviewed and their documentation will be carefully read. This is really important in order to know every patient’s condition, because lupus has many clinical manifestations. Some of the selected patients will have lupus nephropathy and some won’t. Based on this people will be divided into groups one of them composed of healthy volunteers (control group). The work on the project will mainly focus one Ribonucleic acid (RNA) testing. Isolation total RNA from patients’ blood. Deep sequencing and analysis of the levels of expression of different long non-coding RNAs. After the collected data will be analyzed and levels of expressions of different RNAs will be correlated with presence or absence of nephropathy and the activity of the nephropathy.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Students will be able to help with the organization of the work and take part in every possible step. The only activity that will probably present difficulty will be evaluation of the patients’ documentation and actual conversations with them. Everyone will have the opportunity to work with Ribonucleic acid sequencing in a lab with all the newest technology. Ribonucleic acid sequencing is used to reveal the presence and quantity of RNA in a biological sample at a given moment. RNA sequencing involves the following steps – RNA isolation, RNA selection/depletion and complementary Deoxyribonucleic acid synthesis. After the tests are done students will be involved in analysis of the gathered information.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There is none theoretical teaching planned.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
There are no special skills required
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Chau-Ching Liu et al. Biomarkers in systemic lupus erythematosus: challenges and prospects for the future Ther Adv Musculoskelet Dis. 2013 Aug; 5(4): 210–233. doi: 10.1177/1759720X13485503
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