Projects
Name
Epidemiological and pathological profile of thyroid carcinomas at the department of anatomical pathology of Mohammed VI University Hospital of Marrakech.
University
Morocco (IFMSA-Morocco), Faculty of medicine and pharmacy of Marrakech, Marrakech
Domain
Pathology
Departement
Anatomical pathology department, University hospital of Marrakech
Head
Pr. Rais Hanane
Tutor
Pr. Rais Hanane, Dr. Salma Amouzoune and the department residents
Languages
English, French
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Thyroid carcinomas are rare epithelial tumors, representing 1% of the total malignant tumors. They usually manifest as anterior cervical nodules. Their diagnosis, sometimes difficult, is based on the anatomopathological examination of the operative specimen of the thyroidectomy. Thyroid carcinomas have classically good prognosis and present different clinical and evolutionary aspects according to their histological origin. They can occur at any age with two frequency peaks, one between 45 and 50 years old and the other between 65 and 70 years old. Occurring more frequently in women, but mortality is higher in men, probably because of a higher age at the onset of the disease. The incidence, variable across the map, has been increasing markedly in the last 20 years and seems currently stabilized. In contrast, the mortality rate is declining. Therefore, an updated study of their histo-prognostic factors as well as their various epidemiological aspects in comparison with international data is necessary.
What is the aim of the project?
The purpose of our work is to highlight the anatomopathological features and epidemiological aspects of thyroid carcinomas supported by the department of anatomical pathology of Mohammed VI University Hospital of Marrakech, and to update their Histo-prognostic factors while comparing them to other national and international series.
What techniques and methods are used?
- Molecular biology and Immuno-histochemical methods: B-RAF protein Mutation Analysis (Includes testing codons 600 and 601) using Real-time Polymerase Chain Reaction (PCR) so as to Diagnose papillary thyroid carcinoma and assess prognosis, as well as detecting the various described fusion transcripts of PAX8/PPARG (Paired Box 8/Peroxisome proliferator-activated receptor gamma) gene fusion in formalin-fixed, paraffin-embedded thyroid tissue and in fine-needle aspirate biopsy washes from thyroid nodules using reverse-transcription PCR). -Retrospective study of patients' files: The register of the department of pathological anatomy. - Histopathological reports. - The clinical file of the patient including the clinical observation of the patient and the para-clinical examinations.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The incoming student will: -Observe and take part of the pre-analytic phase under supervision of the resident doctors: Reception, Fixation. -Observe and work on biomolecular and immunohistochemical methods under supervision. -Retrospective study and analysis of patients’ files assisted by research PhD doctors. -Work on a standardized report containing all the elements to contribute in a regional registry of thyroid cancer.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, a training on the use of the biomolecular techniques (FISH) will be provided by the head of the departement.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge in molecular biology and oncology is highly recommended.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- Romei; C.; & Elisei; R. (2012). RET/PTC Translocations and Clinico-Pathological Features in Human Papillary Thyroid Carcinoma. Frontiers in Endocrinology; 3. https://doi.org/10.3389/fendo.2012.00054 https://www.frontiersin.org/articles/10.3389/fendo.2012.00054/full
- A thesis of dr. Rajaa Belmaati (in french) : http://wd.fmpm.uca.ma/biblio/theses/annee-htm/FT/2017/these155-17.pdf