Estimation of Prevalence of Hyperhomocysteinemia in healthy medical students ofnJ N Medical College ,Belagavi.
India (MSAI) - Jawaharlal Nehru Medical College/KLE University, Belagavi
Anita teli
Anita teli
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes Yes Yes No No No No
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Currently, hyperhomocysteinemia is a well-known risk factor for variety of vascular diseases. Prevalence of hyperhomocysteinemia increases with age. More than 100 epidemiological, case control and longitudinal cohort studies have established that even mild hyperhomocysteinemia predicts and precedes the development of cardiovascular morbidity and mortality, and is an independent risk factor for cardio- and cerebrovascular diseases Hence, the present study was aimed to investigate the prevalence of hyperhomocysteinemia in healthy medical students in India.
What is the aim of the project?
1. To estimate the prevalence of hyperhomocysteinemia among healthy medical students. 2. To compare the prevalence among males and females students. (No link of hyperhomocysteinemia to vascular disease will be made)
What techniques and methods are used?
An observational, simple blind study will be carried out in healthy medical students (age, 17-24yrs) . Study will be conducted in the department of physiology. All healthy subjects willing to participate will be included in the study. Subjects having any acute or chronic disease were excluded from the study. Ethical clearance will be obtained from institutional ethical clearance committee. Written informed consent will be taken from the all the subjects. Blood samples from fasting subjects will be obtained using EDTA (Ethylenediaminetetraacetic Acid) as anticoagulant by Super Religare Laboratories Ltd, India. Samples will be immediately chilled at <4°C and centrifuged (2000 X g, 10 min) within 1 h of collection. Plasma will be immediately frozen at -70°C until Hcy (Homocysteine) determination. Serum samples will be obtained and stored at -70°C until analysis. Total plasma Hcy levels will be determined by using the Advia Centaur® Immunoassay System (Siemens Healthcare Diagnostics Inc., Tarrytown, NY,USA). Sensitivity and Assay Range was < 0.50 – 65 µmol/L. Statistical methods ANOVA(Analysis of Variance), unpaired t test will be used to compare mean tHcy concentration in association with demographic factors, including age, BMI (Body Mass Index) and gender. Results will be expressed as mean ± Standard deviation/Standard Error of Mean if nothing else is specified.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Data Collection Statistical Analysis Data entry Journal presentation on the related topic Seminar presentation on the related topic Research article writing Going to statistician for statistical work Going to laboratory for assessing the data Going Basic research labs for estimation of values Presenting the final presentation after working.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
yes. 1 hour of teaching / day
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Dental medicine students (IADS members)
- 1.Bhargava S et al. J Clin Biochem Nutr. 2012 50: 222 [PMID: 22573925]