Projects
Name
Celular aspects and molecular biology related to extracelular matrix in cancer
University
Faculdade de Medicina do ABC (FMABC)
Domain
Biochemistry
Departement
Department of Biochemistry, Faculdade de Medicina do ABC, Av. Lauro Gomes, 2000 - Vila Sacadura Cabral, Santo André - SP, 09060-870
Head
Maria Aparecida Pinhal, Thérèse Rachel Theodoro, Carina Mucciolo Melo
Tutor
Maria Aparecida Pinhal, Thérèse Rachel Theodoro, Carina Mucciolo Melo
Languages
English and Portuguese
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Cancer is worldwide known as a multifactor and variable disease. The Department of Biochemistry of FMABC approaches the matter in order to identify the biological relevance in the carcinogenic process, which involves molecules of extracellular matrix. These molecules can help the investigation e maybe can be used as a prevention through tracking programs. The project has different focus methodologies based on molecular biology, biochemistry and pharmacology, the student is able to have a very wide experience because it does have more than one option to focus, giving a more general understanding of the laboratorial production of knowledge.
What is the aim of the project?
To study and analyze molecules associated to oncology processes and cancer itself, prove if it’s possible to use them as markers expression in specific types of cancer
What techniques and methods are used?
The process englobed by the process follow these steps: 1. Extraction of nucleic acids as deoxyribonucleic acid (DNA), microsomal ribonucleic acid (microRNA), messenger ribonucleic acid (mRNA). 2. Amplification reaction: Polimerase Chain Reaction (PCR) and Reverse Transciption, using quantitative methods as SYBR® Green 3. Cell culture manipulation of established lines Analyzation of the processed cells and cell compounds on confocal microscopy, administration of immunohistochemistry and immunocytochemistry methods to refine the results if they’re unsatisfactory after the previous methods use.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be included in all laboratory procedures involved in the project. Starting with basic DNA extraction and refining, also mRNA and microRNA extraction. From this material, the student will be enquired to perform amplification reactions as PCR and reverse transcription reactions to have longer material to work with. Will be included in the tasks cell culture and manipulation, analyzation of the processed cells and cell compounds on confocal microscopy, administration of immunohistochemistry and immunocytochemistry methods to refine the results if they’re unsatisfactory after the previous methods use. The student will develop and design experimental protocols along with the tutor, discuss results with the group, organize the obtained data, construct graphics and tables using basic software as Microsoft Excel, Word and construct presentation using basic Power Point. It is demanded from the student to attend at laboratory meetings of the research group.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, in the beginning of the exchange, as the professor evaluates the student level of knowledge in the matter, it will be provided to him/her activities and complementary readings such as articles and proper bibliography. If it’s necessary, it will be a mini-seminar in presented in English.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student must be interested to learn new methodologies in the research laboratory, to have basic knowledge in biochemistry and cellular biology and be able to work in group Subjects passed: Biochesmistry and cellular biology
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- 1. Theodoro TR; de Matos LL; Sant Anna AV; Fonseca FL; Semedo P; Martins LC; Nader HB; Del Giglio A; da Silva Pinhal MA.Heparanase expression in circulating lymphocytes of breast cancer patients depends onthe presence of the primary tumor and/or systemic metastasis. Neoplasia. 2007;9(6):504-10.