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Evaluation of scaffolds containing the peptide IDR-1002 and the antibiotic Cirpofloxacin for pulp revascularization/regeneration.
Catholic University of Brasilia- UCB
Programa de Pós- graduação em Ciências Genômicas e Biotecnologia
Taia Maria Berto Rezende
Taia Maria Berto Rezende
English or Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Researches in regenerative therapies has been emerging in endodontics, especially in permanet teeth with open apex. The use of an antibiotic triple paste (TAP), containing metronidazole, ciprofloxacin and minocycline can generate microbial resistance, cytotoxicity and dentin discoloration. For this process, synthetic scaffolds allow the incorporation of antimicrobials, growth factors and biomolecules into these systems, which allows the molecule control release and its degradation. Faced with the resistance of several microorganisms, host defense peptides have been studied by It antimicrobial, immunomodulatory and regenerative action. The synthetic peptide IDR-1002 presented in vitro antimicrobial activity in synergism with ciprofloxacin against Enterococcus faecalis and Staphylococcus aureus. Then, this study aims to incorporate the association between IDR-1002 and ciprofloxacin in nanostructures.
What is the aim of the project?
Evaluate in vitro the immunomodulatory activity of polyvinyl alcohol (PVA) nanofibers and collagen hydrogel incorporated with peptide IDR-1002 and the antibiotic ciprofloxacin to determine the potential of this system, aiming the construction of a new product for pulp revascularization/regeneration
What techniques and methods are used?
Researchers selected the host defense peptides and the antibiotic and are now preparing the nanofibre and gel containing both structures. Steps: 1. Host defense peptides selection; 2. Preparation of nanofibre and gel with antibiotic and host defense peptides; 3. Toxicity tests; 4. Immunomodulatory avalidation; 5. In vivo analysis of the effectiveness of the revascularization process (histology analysis of the new tissue).
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- Discuss the project with the post-graduation student; - Read papers related to the project; - Participate in the weekly-lab meetings – These meetings happens all Fridays, 8:30 – 11:30AM; - Participate in the safety lab course; - Practical training with laboratory technicians; - Help with lab work in his/her project – preparing all reagents, processing all experiments and helping in data analysis; - Make a tour of the University; - Interact with all post-graduation students; trying to understand what each one’s work is; - Participate in the week-Post-graduation seminars. These seminars happens every Fridays, 2-3PM.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The safety lab course, week-post-graduation seminars and week-lab meetings. All papers will be provided for the student once he/she will be in the lab.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Scientific interest and discipline Read the group papers Immunology and microbiology knowledge The student should organize all experiments routine to present in our lab meeting in the end of the stage.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Lima; SMF; FREIRE; M. S.; Otim; AL; Cantuária; AP; DUTRA; F. R. P.; Magalhães; B. S.; Sousa; MGC; Migliolo; L.; Almeida; JA; FRANCO; O. L.; Rezende; Taia Maria Berto. Antimicrobial and immunomodulatory activity of host defense peptides; clavanins and LL-37; in vitro: An endodontic perspective. PEPTIDES; v.95; p.16 - 24; 2017.
- Biswaro; LS; Sousa; MGC; Rezende; TMB; Simoni C. Dias; FRANCO; O. L. Antimicrobial Peptides and Nanotechnology; Recent Advances and Challenges. Frontiers in Microbiology; v.9; p.1 - 14; 2018.
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