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Improving Malaria diagnostics in Sudan
Sudan (MedSIN-Sudan) - Khartoum University, Khartoum
Department of Parasitology and Medical Entomology
Dr. Muzamil Mahdi Abdel Hamid; Miss Safa Ahmed
Arabic , English
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Malaria is the major problem in Sudan. Plasmodium falciparum is the major species causing malaria. However, in recent years P. vivax and other species have been involved in the clinical disease in different parts in Sudan. Diagnosis of malaria relies on microscopy and rapid tests, more sensitive methods are required including serology and molecular methods. Molecular methods include Polymerase Chain Reaction (PCR) , Real Time-PCR and DNA sequencing. Serology methods include Enzyme Linked Immuno Sorbent Assay (ELISA) while measuring cellular immunity and cytokine profile by Enzyme Linked Immuno SPOT (ELISpot)
What is the aim of the project?
To evaluate the molecular techniques used in diagnosis of malaria in endemic sites in Sudan.
What techniques and methods are used?
Microscopy; DNA-based method, rapid test and serology. Malaria diagnosis at clinincs; laboratory diagnosis; molecular work; data analysis. Demographic data will be collected using structured questionnaire. Descriptive and infertial statistic will be performed Data will be analysed using SPSS software. Sequencing data will be analysedusing Bioinformatics tools (BLAST, MSA, phylogeny, and MEGA 7.0)
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
History of the patients; signs and symptoms; filling patients record form; lab work Will include design of questionnaire and testing validity, Microscopy, DNA extraction, DNA quantification using nanodrop, PCR and nested PCR, RFLP, and DNA sequencing. In silco and bioinformatics software will be taught.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures by the director of the institute and the head department of Parasitology and Medical Entomology
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Good clinical practice (GCP) and good laboratory practice (GLP)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- - Multiplicity of infection and genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria in Gezira State; Sudan. Parasites & Vectors (2016) 9:362 DOI 10.1186/s13071-016-1641-z - Molecular Evidence of High Proportion of Plasmodium vivax Malaria Infection in White Nile Area in Sudan. Journal of Parasitology Research (2016); Article ID 2892371; http://dx.doi.org/10.1155/2016/2892371
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