Hepatoprotective activity of Kazakhstan endemic plants’ extracts against CCl4 induced acute hepatotoxicity
Kazakhstan (KazMSA) - Astana Medical University, AMU, Astana, Kazakhstan
Department of general and biological chemistry, JSC “Astana Medical University”, Beybitshilik street, 49A,Astana city, Kazakhstan, Z10K9D9
Bolatov Aidos Kanatovich: +77776000096 Department of general and biological chemistry: +7(7172)539537 Department of international cooperation: +7(7172)539442
Tutor, professor – Seitembetov Talgat Sultanovich Bolatov Aidos Kanatovich
Required: English Accepted: Russian, Kazakh
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The liver is a major target for the chemical induced toxicity, as it participates in the metabolism, detoxification, and secretory functions. Liver disease is a worldwide problem and in most of the cases, liver damage is due to oxidative stress. Liver damage, if it is not managed at the right time, may progress from steatosis to chronic hepatitis, fibrosis, cirrhosis and even hepatocellular carcinoma. The active management of liver disease is a major concern in medical science as there are no reliable drugs available in modern medicine. Thus, there is a phenomenal increase in the search for the effective natural products worldwide to treat or to prevent liver toxicity. Keeping up with the pace, we have undertaken the screening of the hepatoprotective activity of the plant material.
What is the aim of the project?
Assess the impact of administration with endemic plant material from Kazakhstan and standard drug silymarin in rats on hepatoprotective activity against carbon tetrachloride (CCl4) induced acute hepatotoxicity.
What techniques and methods are used?
Plant material and extract were developed and provided by JSC “Research and Production Center “Phytochemistry” (Karaganda) under the guidance of academician NAS RK, doctor of chemical sciences, professor S.M. Adekenov. Experimental animals: outbred laboratory rats weighing 180-220 g of male were used. 1. Receiving of the olive oil and test substances; 2. Administration with CCl4; 3. Anaesthetizing using chloroform; 4. Collection of the liver and blood for histological and biochemical analysis respectively 5. Estimation of the liver pathophysiological enzymes such as aspartate transaminase, alanine transaminase, alkaline phosphatase and lipid profile - total cholesterol, VLDL, HDL, triglycerides and bilirubin by using commercially available kits; 6. Evaluation of the lipid peroxidation level by the interacting with thiobarbituric acid; Statistical analysis of the data: analyze for statistically significant differences using one-way analysis of variance (ANOVA) followed by the Tukey-Kramer post analysis test to compare all groups.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will perform all the tasks given by the project, which includes work with biological material, with strict observance of safety measures (wearing masks and gloves during sampling and use of disinfectants). Randomization of laboratory rats, their care and feeding; preparation for the study, introduction of test samples with a probe, administration of drugs; autopsy, under the supervision of a tutor, blood sampling; collection of primary data: rat weight, liver weight, liver macro preparation description; laboratory work on spectrophotometric study of liver enzymes and lipid peroxidation level; The student will conduct a statistical analysis of the data and allocate samples with significant hepatoprotective activity, using one-way analysis of variance (ANOVA) followed by the Tukey-Kramer post analysis test to compare all groups.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures and seminars
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare an abstract
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Skills for procedure with scientific literature, laboratory equipment. Students should have knowledge of general chemistry, biostatistical methods of analysis. Subjects passed: Chemistry, Biochemistry Previous experience with: laboratory work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Anita Singh et al. Clinical Biochemistry of Hepatotoxicity. Singh;J Clinic Toxicol 2011; S:4. doi:10.4172/2161-0495.S4-001;