Projects
Name
Study of antioxidant, antiradical activity and total soluble polyphenol compound in vitro of endemic plant material from Kazakhstan.
University
Kazakhstan (KazMSA) - Astana Medical University, AMU, Astana, Kazakhstan
Domain
Biochemistry
Departement
Department of general and biological chemistry, JSC “Astana Medical University”, Beybitshilik street, 49A, Astana city, Kazakhstan, Z10K9D9
Head
Bolatov Aidos Kanatovich: +77776000096 Department of general and biological chemistry: +7(7172)539537 Department of international cooperation: +7(7172)539442
Tutor
d.ch.s., professor – Seitembetov Talgat Sultanovich Bolatov Aidos Kanatovich
Languages
Required: English Accepted: Russian, Kazakh
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes Yes Yes Yes Yes No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
An antioxidant can be defined as ‘‘any substance that, when present at low concentrations compared with those of an oxidizablesubstrate, significantlydelays or prevents oxidation of that substrate’’. The concept of antioxidant capacity was first originated from chemistry and was later established to biology, medicine, epidemiology and nutrition. Antioxidants are considered important nutraceuticals on account of their many health benefits and are widely used in the food industry as inhibitors of lipid peroxidation. Synthetic antioxidants accumulate in the body causing liver damage and carcinogenesis. Such problems are not seen when natural antioxidants extracted from herbs and spices are used. These extracts are safe, potentially nutritional and have therapeutic effects. On the territory of Kazakhstan there are 5754 species of land plants and among them more than 1500 species of medicinal plants, i.e. the Republic has reserves of useful plants with medicinal properties, a significant part of which is interesting for the study of chemical composition and bioactivity. In the present study, antioxidant and antiradical activity and total soluble polyphenol compound in vitro of endemic plant material from Kazakhstan will be investigated.
What is the aim of the project?
The aim of the work is to study the chemical composition, identification of biologically active polyphenols, as well as the study of antioxidant and antiradical activities of endemic plant material from Kazakhstan by modern spectrophotometric methods.
What techniques and methods are used?
Plant material and extract were developed and provided by JSC “Research and Production Center “Phytochemistry” (Karaganda) under the guidance of academician NAS RK, doctor of chemical sciences, professor S.M. Adekenov. 1. Determination of the total phenolic content of plant material using Folin–Ciocalteu reagent; 2. Evaluation of the antioxidant activity by the ferric reducing antioxidant power assay and methods with o-phenanthroline; 3. Determination of the antiradical activity by the scavenging activity of the stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical; 4. Evaluation of the antioxidant activity by the method with adrenalin. Statistical analysis of the data: analysis for statistically significant differences using one-way analysis of variance (ANOVA).
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will perform all the tasks given by the project, which includes laboratory work on spectrophotometric study of antioxidant and antiradical activities. Calculations and preparation of all necessary reagents; independent work with equipment, under the supervision of a tutor; determination the content of polyphenolic compounds of plant material; conduction of statistical analysis of the data, using one-way analysis of variance (ANOVA) and allocate samples with significant antioxidant and antiradical activities.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures and seminars
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare an abstract
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Skills for procedure with scientific literature, laboratory equipment. Students should have knowledge of general chemistry, biostatistical methods of analysis. Subjects passed: General chemistry, Biochemistry Previous experience with: laboratory work
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- Aparadh V.T. et al. Antioxidative properties (TPC; DPPH; FRAP; metal chelating ability; reducing power and TAC) within some Cleome species. Ann. Bot. (Roma); 2012; 2: 49–56. doi: 10.4462/annbotrm-9958;
- MeenaAfrozeShanta et al. Phytochemical Screening and In Vitro Determination of Antioxidant Potential of Methanolic Extract of StreospermumChelonoides. Journal of Applied Pharmaceutical Science Vol. 3 (03); pp. 117-121; March; 2013. DOI: 10.7324/JAPS.2013.30323