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Methods and techniques for the study and control of schistosomiasis in Pernambuco, northeast of Brazil.
Brazil (IFMSA-Brazil) - Faculdade Mauricio de Nassau, Recife
Laboratorio e Servico de Referência em Esquistossomose. Department of Parasitology. Av. Professor Moraes Rego, Cidade Universitaria, CEP 50.740-465. Recife, Pernambuco - Brazil.
Wheverton Ricardo Correia do Nascimento
Wheverton Ricardo Correia do Nascimento
English, Spanish and Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Schistosomiasis is an endemic disease in Brazil and causes high rates of morbimortality. The transmission of helminth Schistosoma mansoni occurs by contact with contaminated water with cercariae, and has been countered by malacological and epidemiological surveys and case management. However, it has not been enough to control this endemic disease. The infection is characterized in the acute and chronic phases. Hepatosplenic is the most severe clinical condition, and it may cause hepatosplenomegaly, ascites, digestive bleeding and death of patients.
What is the aim of the project?
To evaluate the epidemiological situation and the effectiveness of the schistosomiasis control program in Pernambuco
What techniques and methods are used?
It will be used techniques for parasitological diagnosis as: The Kato-Katz technique will be performed, with collection of feces, fresh, in universal collector and processing, in laboratory, within 24 hours. All material will be examined IFMSA International Secretariat, c/o IMCC, Nørre Allé 14, 2200 København N., Denmark in general in this project?) under the microscope and the eggs will be counted. The malacological diagnosis will use snail dissection methods for species identification, light exposure and crushing technique, recommended by the World Health Organization for the control of the disease. Then, Nested PCR (Polymerase Chain Reaction) will be used to diagnose Mansoni schistosomiasis in vector molluscs After all, the techniques of GIS, the Geographic Information System, will be used to provide a risk perspective to generate models for the transmission of schistosomiasis in order to assess the same diagnosis of its risk factors.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student should follow some research steps: Accomplish the coproparasitological evaluation of the samples collected in the research, from the preparation of the slide to its microscopic IFMSA International Secretariat, c/o IMCC, Nørre Allé 14, 2200 København N., Denmark observation. For this step, the student will use the Kato-Katz method. Accomplish the malacological and molecular diagnosis, through the dissection of the snails and their exposure to light, besides subjecting the vectors to the PCR technique to identify the parasite. Help in tabulating the data obtained and using the GIS Geographic Information System, to make a geoprocessing, and thus understand the dynamics of diseases and their variations in space and time.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
It is suggested that the student study previously the symbiotic and infection cycle of Schistosoma mansoni. In addition, he will participate in scientific discussions with the laboratory staff.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report - The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge to produce a summary to send to a congress
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Teodosio VT; Do Nascimento WR; Nobrega CO; Melo TE; Araujo G; Fernandes ES; Goncales JP; Medeiros D; Barbosa CS; and De Souza VO. Investigation of the positivity profile for the skin prick test in children infected with parasites in the metropolitan region of Pernambuco; Northeast of Brazil. World Allergy Organization Journal20158(Suppl 1):A241 DOI: 10.1186/1939-4551-8-S1-A241
- Gomes EC; Leal-Neto OB; de Oliveira FJ Jr; Campos JV; Souza-Santos R; Barbosa CS. Risk analysis for occurrences of schistosomiasis in the coastal area of Porto de Galinhas; Pernambuco; Brazil. BMC Infect Dis. 2014 Feb 23;14:101. doi: 10.1186/1471-2334-14-101.
- Barbosa CS; Barbosa VS; Nascimento WC; Pieri OS; Araujo KC. Study of the snail intermediate hosts for Schistosoma mansoni on Itamaraca Island in northeast Brazil: spatial displacement of Biomphalaria glabrata by Biomphalaria straminea. Geospat Health. 2014 May;8(2):345-51.
- Leal Neto OB; Gomes EC; Oliveira Junior FJ; Andrade R; Reis DL; Souza-Santos R; Bocanegra S; Barbosa CS. Biological and environmental factors associated with risk of schistosomiasis mansoni transmission in Porto de Galinhas; Pernambuco State; Brazil. Cad Saude Publica. 2013 Feb;29(2):357-67.
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