Projects
Name
Investigation on 1) primary tubular disorders and 2) Growth plate in chronic kidney disease and X-linked hypophosphatemia
University
Spain (IFMSA-SPAIN) - University of Oviedo, Oviedo
Domain
Nephrology
Departement
Department of Medicine. School of Medicine. c/ Julian Claveria 6, 33006 Oviedo, Spain
Head
Laura Alonso, Helena Gil-Peña, Fernando Santos
Tutor
Laura Alonso, Helena Gil-Peña, Fernando Santos
Languages
English, Spanish
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes Yes Yes No No Yes Yes No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Primary tubular disorders are rare diseases. Our group is leading an international collaborative effort (www.renaltube.com) aimed at a better knowledge of genetic basis and the clinical phenotype of several tubulopathies including distal renal tubular acidosis, primary hypophosphatemias and Gitelman’s syndrome. The analysis of growth cartilage using animal models of growth retardation secondary to chronic kidney diseases such as uremia and X-linked hypophosphatemia is used by our group to investigate the pathogenic mechanism of growth impairment in these diseases. This study requires animal models because of the impossibility to obtain growth plate samples in humans.
What is the aim of the project?
Training in lab basic techniques focused on the study of projects related with the line of research of the group.
What techniques and methods are used?
In addition to general lab techniques, the following technical tools are used for the development of the project: histological examination of growth plate and bone samples; immunohistochemistry in growth plate to detect the expression and localization of proteins involved in the metabolism of chondrocytes; confocal microscopy to analyze shape, size and density of growth cartilage chondrocytes. Techniques of PCR and gene sequencing will be used to find mutations in DNA extracted from patients with X linked hypophosphatemia. The student will be involved in the above techniques mostly in the preparation of laboratory buffers, immunohistochemistry, computerized quantitative analysis of protein expression, PCR and, if possible statistical analysis of data. The particular involvement in these techniques cannot be defined now because it will depend on the period of stay and the stage of projects’ development.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
In addition to general lab techniques, the following technical tools are used for the development of the project: histological examination of growth plate and bone samples; immunohistochemistry in growth plate to detect the expression and localization of proteins involved in the metabolism of chondrocytes; confocal microscopy to analyze shape, size and density of growth cartilage chondrocytes. Techniques of PCR and gene sequencing will be used to find mutations in DNA extracted from patients with X linked hypophosphatemia. The student will be involved in the above techniques mostly in the preparation of laboratory buffers, immunohistochemistry, computerized quantitative analysis of protein expression, PCR and, if possible statistical analysis of data. The particular involvement in these techniques cannot be defined now because it will depend on the period of stay and the stage of projects’ development.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Bibliografphy and direct explanatios. Attendance to research seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Willingness and dedication
Are there any legal limitations in the student’s involvement
Yes
Insurance and authorization of the institution (LOREs will tell by the time of sending the CA)
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Fernández-Iglesias Á; López JM; Santos F. Growth plate alterations in chronic kidney disease. Pediatr Nephrol. 2018 Dec 14. doi: 10.1007/s00467-018-4160-7. [Epub ahead of print] PubMed PMID: 30552565.
- Fernández-Fernández M; Gil-Peña H; Coto E; García Pérez N; Santos F. Salt-losing tubulopathy and chronic dermatitis. Kidney Int. 2018 Aug;94(2):433. doi: 10.1016/j.kint.2017.12.007. PubMed PMID: 30031451.
- Fuente R; Gil-Peña H; Claramunt-Taberner D; Hernández- Frías O; Fernández-Iglesias Á; Hermida-Prado F; Anes-González G; Rubio-Aliaga I; Lopez JM; Santos F. Marked alterations in the structure; dynamics and maturation of growth plate likely explain growth retardation and bone deformities of young Hyp mice. Bone. 2018 Nov;116:187-195. doi: 10.1016/j.bone.2018.08.004. Epub 2018 Aug 8. PubMed PMID: 30096468.
- Alonso-Varela M; Gil-Peña H; Coto E; Gómez J; Rodríguez J; Rodríguez-Rubio E; Santos F; RenalTube Group. Distal renal tubular acidosis.