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Evaluation of the effect of three medicinal plants extracts and fractions over stem and neoplastic cells of gastric cancer.
Universidad Cientifica del Sur
Chemistry and Biochemistry of Natural Products lab. Cell Culture lab. Universidad Científica del Sur. Panamericana Sur Km 19, Villa I. Villa El Salvador. Lima-Peru
Dr. Jose Amiel Perez
Dr. Alvaro Marcelo Rodriguez
Type of Research Project
- Basic science
What is the background of the project?
Currently, gastric cancer is the second most frequent neoplasm regardless of sex in Peruvian population, representing 11.1% of all cases. It is usually diagnosed in advanced stages, presenting an unspecific symptomatology such as weight loss, dysphagia, upper gastrointestinal bleeding, anemia and persistent vomiting. Therefore, native Peruvian plants are being used for research to provide new alternative ways of treatment. An experimental analytical study where Buddleja species incana globosa and Dracontium spruceanum can be found is being developed with the purpose of evaluating if there is an apoptotic effect of the chromatographic fractions extracted from these plants over neoplastic cell and stem cells. This will contribute valuable information to increase the research topic about cancer treatment.
What is the aim of the project?
To evaluate the effect of the extract and chromatographic fractions of three medicinal Plants: Buddleja incana, Buddleja globosa and Dracontium spruceanum, over the stem cells of gastric cancer.
What techniques and methods are used?
Chromatographic fractions will be obtained from the elaboration of medicinal plants extracts to be categorized phytochemically. Stem cells of gastric cancer will be isolate and categorize using flow cytometry with the technique of 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (The CyQUANT XTT Cell Viability Assay). First, the cells will be collected by centrifugation at 200 x g for 10 minutes. Then, after a few steps of centrifugation and dilutions, the cell culture is obtained. The XXT protocol is divided in 8 steps, in which the cell culture is incubate and the XTT Reagent is added. Finally, the absorbance of all the assay wells is measured.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Knowledge about the elaboration of extracts of medicinal plants. Learn the application of medicinal plant extract in cancer cells. Review and domain of flow cytometry. Knowledge about the fluorescence and spectrophotometry equipment to measure cell viability. Learn to identify the effect of medicinal plants on cancer cells. The student will be able to do workshop lab as extracting samples from gastric tumor biopsies. If the student performs correctly, the tutor will let him or her manipulate the laboratory instruments and samples, otherwise, the student will work under the others students suprevisions in charge.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Bibliographic search Genetic regulation Hormonal signaling pathways Cell proliferation Primary Gastric tumors In vitro cells culture Cell lines previous experience
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Teal E; Steele NG; Chakrabarti J; Holokai L; Zavros Y. Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration. J Vis Exp. 2018;(135).
- Schumacher MA; Aihara E; Feng R; Engevik A; Shroyer NF; Ottemann KM; et al. The use of murine-derived fundic organoids in studies of gastric physiology. J Physiol. 2015;593(8):1809-27.
- Bartfeld S; Clevers H. Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori. J Vis Exp. 2015;(105):1-9.
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