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Molecular epidemiology of infections caused by multidrug-resistant Gram negative bacilli in Joinville, Southern of Brazil: Cross-sectional transmission between animals, pets and hospitalized patients
Universidade da Regiao de Joinville (UNIVILLE)
Molecular Biology Laboratory, Medicine Dept. Paulo Malschitzki Street, 10 – Industrial Zone, Zip Code 89219-710, Joinville SC Brazil
Paulo Henrique Condeixa de França
Paulo Henrique Condeixa de França
English, Portuguese and Spanish
Type of Research Project
- Basic science
What is the background of the project?
Healthcare-associated infections (HAI) often pose a major public health problem worldwide. Currently, the most affected population are critically ill patients in ICUs, affecting up to 70% of the total costs of admissions in these units. The great diversity of genetic elements conferring antimicrobial resistance in Gram negative bacilli has encouraged the expansion of local studies on such determinants of resistance. The study of molecular determinants of resistance and spread of resistant clones in ICUs, coupled with monitoring the clinical course of critically ill patients in public hospitals in Joinville, the most populous city of Santa Catarina, are of great importance for establishing the epidemiological profile of HAI, the identification of possible clonal spread between the hospitals and the evaluation of the impact of multi-resistant bacteria in clinical outcome and length of stay in the ICU.
What is the aim of the project?
To investigate the occurrence and spread of strains carrying genes coding for metallo-β-lactamases and serine-β-lactamases.
What techniques and methods are used?
Basic procedures of Molecular Biology as: Extraction and purification of bacterial DNA Quantification assessment by spectrophotometry Standard and Multiplex Polymerase Chain Reaction for the investigation of genetic markers of antimicrobial resistance. The information obtained with the mentioned procedures above will be organized in Microsoft Excel tables and crossed with other studies information to evaluate the best course of treatment to be implemented.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be in charge of all laboratory procedures required. The student will perform deoxyribonucleic acid extraction and purification through polymerase chain reaction (PCR) technique. The student will also do quantification of cells compounds by spectrophotometry. Following the tests, the student will perform standard and multiple PCR to investigate genetic markers of antimicrobial resistance and possible evaluate possible changes in DNA that could modify the course of antimicrobial resistance. The performance of technical procedures and interpretation of the results will be done under monitoring at the laboratory by the tutor and will be discussed with the group.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No. Weekly reunions with the whole staff are performed in Portuguese, but separated discussions among tutors and foreign student will be held during the stay.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basics of good laboratory practices. Be interested, polite and proactive. The student must be vaccinated for hepatitis B.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Students in biomedical fields
- Antimicrobial resistance profiles and oxacillinase genes in carbapenem-resistant Acinetobacter baumannii isolated from hospitalized patients in Santa Catarina; Brazil. Rev Soc Bras Med Trop. 2015 Nov-Dec;48(6):699-705. doi: 10.1590/0037-8682-0233-2015.
- The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting. Rev Soc Bras Med Trop. 2016 Jul-Aug;49(4):433-40. doi: 10.1590/0037-8682-0002-2016.
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