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Prostate cancer progression –epithelial mesenchymal transition in prostate tissues and cell culture
Poland (IFMSA-Poland) - Jagiellonian University, Krakow
Chair of Medical Biochemistry
prof. Piotr Leidler PhD
Joanna Dulińska-Litewka PhD
Type of Research Project
- Basic science
What is the background of the project?
Prostate cancer (PC) is a hormone-regulated tumor and one of the leading cause of cancer-related deaths among men. The most common therapy is androgen-deprivation therapy involving anti-androgen treatments and/or castration therapy. Initially, PC is regulated by androgens activating the androgen receptor (AR) and subsequently progresses to aggressive androgen-independent stage. The mechanisms responsible for transforming androgen-dependent PC into its androgen-independent form have not been well understood. Malignant transformation is characterized by the E- to N-cadherin switch leading to increased tumor invasiveness and cancer progression. Shift in cadherin profiles acts as a major hallmark of the epithelial–mesenchymal transition (EMT) and is associated with increased expression of vimentin as well as production of transcription factors (Snail, Twist) which inhibit E-cadherin.
What is the aim of the project?
The aim of the project is to understand the mechanisms responsible for progression of PC from androgen-dependent to androgen-independent form.
What techniques and methods are used?
During project the prostate tissues cells will be isolated from the postoperative material provided by the Department of Patomorphology in the University Hospital in Krakow. Separated cells will later become the start of cell cultures to increase the number of samples. Afterwards each cell culture will be analyzed at the mRNA level using PCR(polymerase chain reaction) technique to look for the mutations responsible for responsible for transforming androgen-dependent PC into its androgen-independent form. At the end the results will be compared with the clinical data and results from other laboratories, with one looking for the correlation between the mutations and the clinical symptoms.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
During project student is expected to learn how to do following things: - perform RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) and Western Blot experiments - protein sample preparation, - run the cell culture in hypoxic and normoxic conditions Students are also expected to learn theoretical bases for cell culturing: culture conditions, suitable vessels (flasks, roller bottles, multi-well plates, medium with nutrients, growth factors, hormones, and gases), the physicochemical environment (pH, osmotic pressure, temperature).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
short lectures given by the tutor at the start of the exchange, possibility of taking part in extra seminary classes – short (30 min) lectures at the beginning of every day
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic laboratory skills: PCR Theoretical basics about hormonal regulation of prostate gland
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Mohammad Imran Khan; Abid Hamid; Vaqar Mustafa Adhami; Rahul K Lall;Hasan Mukhtar; Role of Epithelial Mesenchymal Transition in Prostate Tumorigenesis. Current Pharmaceutical Design (2015); vol. (21/10) doi: 10.2174/1381612821666141211120326
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