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Isolation of new probiotic microorganisms with potential for use in biomedicine and biotechnology
Slovakia (SloMSA) - University of Pavol Jozef Safarik, Košice
Institute of Experimental Medicine
MVDr. Alojz Bomba, DrSc.
MVDr. Alojz Bomba, DrSc. , Mgr. Ľuboš Ambro, PhD.
English , Slovak
Type of Research Project
- Basic science
What is the background of the project?
Probiotic microorganisms are widely used in preventive and supportive biomedicine and in food biotechnology. The use of new probiotic strains in preclinical and clinical experiments and in practice requires comprehensive and detailed analysis of the strains at phenotypic, biochemical and molecular level. There are various mechanisms by which probiotics exert their beneficial effects: regulation of intestinal permeability, normalisation of host intestinal microbiota, improvement of gut immune barrier function, and adjustment between pro- and anti- inflammatory cytokines. Hence the effect is often strain specific, isolation of novel protiotic strains is still desirable.
What is the aim of the project?
The proposed project aims at isolation and characterization of new probiotic strains of the Bifidobacterium and Lactobacillales taxons. We propose that these new isolated strains could have unique beneficial properties so they could be useful in biomedicine and biotechnology.
What techniques and methods are used?
Microbial cell culture: - Cultivation media preparation - Cultivation of bacteria using selective media Microbe identification: - mass spectrometry (MALDI-TOF) based identification Microbial DNA isolation: - DNA extraction using a kit DNA quality check: - DNA electrophoresis - DNA concentration quantitation using fluorimeter
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Searching and selection of scientific literature – use of The National Center for BiotechnologyInformation (https://www.ncbi.nlm.nih.gov/) Basic lab work – preparation and sterilization of solutions and cultivation media Microbial cell culture – cultivation of bacteria on basic / selective media, basic streaking and dilution techniques, cell counting Microbe identification – MALDI-TOF analysis Microbial DNA isolation – isolation using commercially available kit for genomic DNA, quality check using DNA electrophoresis Data analysis, problem solving and discussion
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Shokryazdan P et al. Probiotics: From Isolation to Application. J Am Coll Nutr. 2017 Nov-Dec;36(8):666-676. de Vrese M; Schrezenmeir J. Probiotics; prebiotics; and synbiotics. Adv Biochem Eng Biotechnol. 2008;111:1-66. Fontana L et al. Sources; isolation; characterisation and evaluation of probiotics. Br J Nutr. 2013 Jan;109 Suppl 2:S35- 50.
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