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Metric Characterisation and Disease Correlated Evaluation of Immuno-histologic Markers Expression in Tissue Slices
Slovenia (SloMSIC) - University of Maribor, Maribor
Institute for Anatomy, Histology and Embryology. Medical Faculty, University of Maribor
dr. Miha Munda, DVM
English, German, Croatian, Serbian, Slovenian, Russian-for conversation purposes only
Type of Research Project
- Basic science
What is the background of the project?
Molecular immuno tissue markers are of limited use in histopathology diagnostics, because not enough research data is available and thus no standardization. The tissue immuno techniques are helpful in detecting specific cell types, or intercellular matrix when in doubt. But the diagnostic and research potentials of immune detectable tissue markers are far beyond today’s use. There are unused potentials, as some studies showed, that correlations between tissue immuno markers and disease stage or other patient’s data could lead, at least, towards better disease prognostics. That for new basic approaches and technologies have to be discovered.
What is the aim of the project?
The aim of this research is to improve detecting techniques and calculative approaches of molecular tissue markers evaluation and the linkages to the disease symptoms and other clinical data.
What techniques and methods are used?
-Newer immuno-histologic staining reactions and adapted imunohistochemical protocols in terms of polymer bounded antibodies. Lab work like pipetting, preparing of reagents, applying of reagents. Manipulating tissue slices, weighing of reagents. -Detecting staining areas via microscopy. Evaluating of staining intensity, area and semi-automatic cell counting, followed by cross-calculations. -Taking images with computer supported microscope camera. -Computer software assisted image analyzing. -Writing reports and short summaries with computer.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
After the theoretical education phase on each of the steps described in the section about techniques and methods above, the student is expected to do his or her own experiments by him or herself. The mentor is available for consultations at all times. The student should write down all the procedures and make notes (lab protocols) about the results and each step that was performed. In case of unsuccessful experiment, student should try to find out and correct the error, then repeat the experiment.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be a textbook as well as lab procedures demonstration provided to the student. For better understanding, it is expected from the candidate, that she/he is familiar with basic immuno-staining techniques in the theory, and working with computers.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed - Cell Biology; Previous experience with general simple laboratory manual work, microscoping, pipetting, average work on computer, writing in MS-Word or Libre Office – Write.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Krstanoski Z; Kokalj Vokac N; Zagorac A; et al: TMPRSS2:ERG gene aberrations may provide insight into pT stage in prostate cancer. BMC Urology 16(1):35. Doi: 10.1186/s12894-016-0160-8
- Munda M; Daris B; Hajdinjak T; et al: Morphometrical analysis of the p53 tissue marker in prostatic adenocarcinoma and its relationship to Gleason score and PSA level. Acta medico-biotechnica 2013; 6(1): 59-64.
- Stiblar-Martincic D; Munda M; Hajdinjak T; et. al: Comparison of microvessel density and PSA level in prostatic adenocarcinoma of Gleason score 6 and 7. Acta medico-biotechnica 2012; 5(1): 54–60.
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