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Development of Delivery Vectors for Physiologically Active Compounds via Cell Penetration Peptides
KMSA (Korea) - Konyang University, Daejeon
Department of Anatomy
Do Kyung KIM
Do Kyung KIM
Type of Research Project
- Basic science
What is the background of the project?
The ultimate goal of this study is to develop a delivery system for physiologically active substances, such as protein and genetic materials, and its efficient development of magnetofection system to enhance the efficacy of transfection using cell penetration peptide (CPP), biodegradable synthetic polymer, and superparamagnetic nanoparticles. The delivery system is composed of dendrimer-based biocompatible materials. It can incorporate physiologically active substances inside the molecules and covalently bind CPP on the surface of dendrimer, maximizing the transfer of physiologically active substances into cells, and solving the problem of magnetofection using a conventional method. In order to maximize the efficiency of transfection by transferring physiologically active substances into the target cells, a novel concept of Magnetofection system using electromagnet is introduced by combining the high-speed rotation and vibration of particles.
What is the aim of the project?
We will develop novel gene transfection agent based on linear polyethylene imine (LPEI). LPEI will be modified with several different molecules such as d-mannose, cholesterylchloroformate and cell penetration peptide, etc. to enhance/improve the gene transfection efficiency.
What techniques and methods are used?
During the course of this research, students will basically learn the conjugation technique by chemical reaction of organic compound and target substances, and important points to consider in chemical reaction. Finally, we will learn the basic mechanisms of formation of chemical structure and principle of gene transfection agents, and learn more about instrumentation techniques and data analysis required for research purpose. Training students who participate in this course will concentrate on the synthesis of gene transfection agents, transfection efficiency measurement and data analysis techniques, and ultimately aim to produce and publish scientific papers.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students participating in this research project will conduct research focusing on short-term research because of their relatively short participation period. For example, we will construct a gene transfection agents using a chemical synthesis techniques and observe the gene transfection efficiency using a cell for the confirmation of efficacy using a confocal microscope. Final results will be published as a short paper. Student will be involved seriously both experiment and writing the paper.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The professor(Do Kyung Kim) will provide serious assistance while the student is studying. In the form of helping students, personal teaching is carried out in parallel with paper reading and lecture. In addition, if the student requires help, the professor will take part in the full experiment directly, and the whole experiment regarding the project will be supported.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Chemistry, Previous experience with: Laboratory work, but not obligatory
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- J. Shin; Y. Yin; H. Park; S. Park; U.L. Triantafillu; Y. Kim; et al.; p38 siRNA-encapsulated PLGA nanoparticles alleviate neuropathic pain behavior in rats by inhibiting microglia activation; Nanomedicine; 13(2018) 1607-21.
- K.S. Yu; J.Y. Oh; M.C. Kim; S.H. Kang; N.S. Lee; S.-Y. Han; et al.; Controlled Release of Ursodeoxycholic Acid from Pullulan Acetate Nanoparticles to Modulate Glutamate-Induced Excitotoxicity in PC-12 Cells; Journal of Nanomaterials; 2018(2018) 12.
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