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Identification of Isolated Bacteria from the Mangroves
Brazil (IFMSA-Brazil) - Universidade Tiradentes (UNIT), Aracaju
Laboratory of Molecular Biology (LBM) of the Institute of Technology and Research. Instituto de Tecnologia e Pesquisa, Av. Murilo Dantas, 300, 49032-490
Luciana Maria de Hollanda
Luciana Maria de Hollanda
English, Spanish and Portuguese
Type of Research Project
- Global Action Project (GAP)
What is the background of the project?
Alongi has made some interesting observations about microbial communities in mangrove sediments. We read that the total microbial biomass is never greater than 1.2% of the total mass of detritus. Bacteria and fungi make up about 91% of the total microbial biomass, while algae and protozoa represent 7% and 2%, respectively in these ecosystems. Mangrove sediments, owing to their peculiar characteristics, have long since caught the attention of researches exploring bacterial diversity. Jaitly and Rai suggest that mangrove sediments should be explored for biotechnology applications and highlighted thermophilic and thermotolerant bacteria as good targets. High temperatures, high levels of salinity, high pH, high levels of organic matter, low aeration and moisture provide interesting substrate conditions conducive to the development of diverse bacteria, with biotechnology potential.
What is the aim of the project?
Identify possible bacteria present in soil samples of the mangroves of Aracaju - Sergipe that have some biological activity.
What techniques and methods are used?
This project comprises the extraction, amplification and identification of different bacteria lineages present in samples gathered from the mangroves in Aracaju/SE. These samples were made available by the Laboratory of Molecular Biology (LBM) from the Institute of Technology and Research. The technique used for this project will consists in sequencing the coding segment of subunit 16S from rRNA. Both extraction and amplification steps will be executed by the researchers at LMB. The sequencing step will be executed at Campinas State University (UNICAMP). The sequences will be compared to data from GenBank and identified according to family, genus, and species at the databank Silva 16S. The data gathered will be organized in scientific articles and abstracts to be presented at national and international congresses during the final steps of the project.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student should learn how to perform DNA extraction using the Aaronson process for three days. Then, the student will remove an aliquot of each pure colony by homogenizing a sample in 0.2 ml eppendorfs containing 15 μl of sterile ultrapure water (water for injection). The samples will be subjected to a temperature of 95 °C for 15 minutes in an equipment thermal cycler for a cellular leisure trip. In addition, the student will learn how to perform a PCR, obtaining a name of the 16S rRNA region sequencing identity. In sequence, the PCR products will be applied in agarose gel 1%, to verify the presence of the samples. After screening, the amplification products are sent to the sequencing performed on Macrogenesis (Macrogênio, Inc., Seoul, Korea) and as sequences compared to the GenBank (National Biotechnology Information Center - NCBI, 2016) database
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Basic of microbiology and molecular biology.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Curiosity, proactive and being committed towards the project. Besides that, it is required writing skills, especially about scientific articles.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Ravikumar; S.; Kathiresan; K.; Ignatiammal; S. T. M.; Babu Selvam; M.; and Shanthy; S. (2004). Nitrogen-fixing azotobacters from mangrove habitat and their utility as marine biofertilizers. J. Exp. Mar. Biol. Ecol. 312; 5–17. doi: 10.1016/j.jembe.2004.05.020
- Edwards; K.J.; Johnstone; C and Thompson; C.1991. A rapid and simple method for the preparation for plant genomic DNA for PCR analysis. Nucleic Acids Research 19; No.6: 1349.
- Jaitly; A.K.; Raí; J.N. (1982). Termophilic and thermotolerant bacteria isolated from mangroves swamps. Mycol.(74); 1021-1022.
- Alongi; D.M. (2002). Present state and future of the world's mangrove forests. Environ. Conser. 29 (3); 331-349
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