How the stimulation of olfactory neurons affects the metabolism of amyloid proteins in mice.
Poland (IFMSA-Poland) - Nicolaus Copernicus University Ludwik Rydygier Collegium Medicum, Bydgoszcz
Department of Molecular Cell Genetics
Rafal Butowt, PhD
Rafal Butowt, PhD
english, spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Neural network activity is a fundamental phenomenon affecting processes such as learning and memory, and thus the behavior of animals and humans. It is known that external factors affect the activity of various groups of neurons in the brain. On the other hand, the activity of individual neurons alone can regulate or modulate the metabolism and / or function of certain proteins. In the study of memory and learning, the hippocampus model is most commonly used. However, an alternative model may also be a model of olfactory neurons because these neurons also proliferate and show a similarly high degree of plasticity. The proposed project plans to use this model to analyze the impact of olfactory neuron stimulation on the qualitative and quantitative process of regulated proteolysis of the Amyloid precursor protein (APP) protein and its homologues. Basic biochemical and cell biology techniques will be used for this purpose. Recent studies show that these proteins play an important modulatory role in nerve synapses, and that various fragments of this protein can play completely different physiological functions.
What is the aim of the project?
Investigation of changes in the level of APP family proteins after sensory stimulation of olfactory neurons in mice.
What techniques and methods are used?
- Non-invasive in vivo stimulation of mice with a mixture of different odorants. - Preparation of total and soluble protein samples from selected mouse tissues. - Analysis of amyloid proteins by Western blotting and immunocytochemistry.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
During this project student is expected to learn how to do following things: protein sample preparation, total protein quantification, perform Western Blot experiments, sodium dodecyl sulfate–polyacrylamide gel electrophoresis and microscopy analysis of protein sample. Also conduct analysis of amyloid protein sample using immunocytochemistry. Perform non-invasive in vivo stimulation of mice with a mixture of different odorants.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Theoretical information regarding the subject under examination will be provided to the student by the tutor (RB).
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic molecular and cell biology skills (basic protein work such as sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, usage of light microscopy, preparation of chemical solutions, etc). The student has to have passed following subjects: general biology and/or biotechnology knowledge
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Login H; Butowt R; Bohm S. 2015. Activity-dependent and graded BACE1 expression in the olfactory epithelium. Brain Struct Funct Jul;220(4):2143-57.