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The role of HIF-1 transcription factor in the progression of Multiple Myeloma
Poland (IFMSA-Poland) - Jagiellonian University, Krakow
Chair of Medical Biochemistry ul. Kopernika 7 31-034 Kraków
Prof. dr hab. Piotr Laidler MD, PhD
dr Kinga Kocemba-Pilarczyk PhD
Type of Research Project
- Basic science
What is the background of the project?
Multiple myeloma (MM), one of the most common hematological malignancies in adults, is characterized by a clonal expansion of malignant plasma cells in the bone marrow (BM), associated with suppression of normal hematopoiesis, renal failure and osteolytic bone lesions. Recently, it was described that myeloma cells are chronically exposed to hypoxic environment in the BM. This is supported by several studies, demonstrating the stabilization and nuclear localization of HIF-1 alpha protein (Hypoxia-inducible factor 1-alpha) in malignant plasma cells. While numerous research groups have examined the role of hypoxia in solid tumors progression, confirming its critical role in this process, there is a limited number of published data regarding the functional role of hypoxia and the HIF-1 transcription factor in the hematological malignancies, such as. MM. Thus, in the frame of the project the role of hypoxia and HIF-1 transcription factor will be examined.
What is the aim of the project?
The aim of the project is to explore the issue of hypoxia in MM, by analyzing the global influence of hypoxia/re-oxygenation cycles on the malignant plasma cells.
What techniques and methods are used?
In this project RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) is used: In RT-PCR, the RNA template is first converted into a complementary DNA (cDNA) using a reverse transcriptase. The cDNA is then used as a template for exponential amplification using PCR. Also Cell culture techniques and analysis of transcriptome databases are used. Western Blot method: the sample undergoes protein denaturation, followed by gel electrophoresis. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognises and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
During project student is expected to learn how to do following things: read, analyze and prepare the data, perform Reverse Transcriptase-Polymerase Chain Reaction and Western Blot experiments, run the cell culture in hypoxic and normoxic conditions. Student is also expected to work with literature which will be given by the tutor at the beginning of the exchange. At the end of the exchange it is expected to present data of the project as a result of prepared documents from the whole exchange.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
general knowledge about the role of hypoxia in progression of cancer, short lectures given by the tutor at the start of the exchange.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields - Dental medicine students (IADS members)
- Hypoxia and metabolic adaptation of cancer cells K L Eales; K E R Hollinshead & D A Tennant Oncogenesis (2016)
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