Relationship between differentiated thyroid cancer and Hashimoto thyroiditis – Oxidative stress profile and immunological response
Universidade Estadual de Londrina (UEL)
Department of General Pathology. PR445 Km380 Campus Universitário – Universidade Estadual de Londrina, Londrina – PR, Brazil. CEP 86057-970
Prof. Dr. Fabio Henrique Kwasniewski
Profa. Dra. Alessandra Lourenço Cecchini Armani
English, Spanish and Portuguese
8 weeks
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
During the past few decades, the incidence of thyroid cancer has increased substantially in many countries, being papillary thyroid cancer (PTC) the most predominant. Adding to that figure, Hashimoto thyroiditis (HT) is considered the most common thyroid autoimune disease. The synchronous occurence of HT and PTC indicates an immunological link between the two entities. Because of the conflicting reports on the subject, without any benefit reaching directly to the patient, the aim of this study is to make a comparative investigation on the participation of chemical mediators of inflammation and oxidative stress in patients with PTC and HT, and the association between these two diseases, and confronting those results to patients with benign pathologies.
What is the aim of the project?
The objective of the present work is to make a comparative investigation on the participation of chemical mediators of inflammation and oxidative stress in patients with TPC and HT, and the association between these two diseases, comparing with patients with benign pathologies. The comparison will be performed in tissue microenvironment (thyroid) and systemically (blood).
What techniques and methods are used?
A fragment containing part of the tumor and part of non-neoplastic tissue will be separated from the surgical piece as soon as the piece is removed, it will be immediately stored on ice and then sent to be processed for oxidative and nitrosative stress screening using immunohistochemistry techniques for p53, Nrf2 and TGF-β1. The following inflammatory parameters will be evaluated in the blood: C-reactive protein (CRP), γ-glutamyltranspeptidase, uric acid, TNF-α, TGF-β1, IL-1β, IL-16, IL-4, IL-5, IL -10, IL-17, INF-g, for the characterization of the TH1, TH2, TH0 or TH17 response. For the systemic oxidative stress assays, total antioxidant capacity (TRAP), catalase enzyme activity and superoxide dismutase (SOD), glutathione, malondialdehyde (MDA) and total thiol levels will be analyzed through laboratory measures results. They will use a self-created program personalized to group the results acquired, following the results will be crossed with the one from similar studies by the study group to analyze how satisfying was the outcome.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be involved in the invitation to the patient to participate in the study, as well as in the application of the informed consent term. The student can (if wish to) accompany the surgeries for the extraction of the sample (observation). Students will be able to process patients' blood samples after receiving good practice training for biological sample handling and safe handling of biological samples. The student will be able to learn how to process the samples and make the laboratory and histopathological analyzes. Finally, the student will be able to learn the process of choosing the most adequate statistical analysis for each method used. Every step of the research the student can get involved if they feel comfortable. Therefore, he will also be able to participate in scientific seminars on the subject.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
At the start of the work in the project the student will receive scientific articles and will participate in periodic seminars on the project. The student will be able to ask questions to the professors and graduate students involved in the research.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
None. Subjects passed: Pathology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- SADANI; G.R.; NADKARNI; G.D. Role of tissue antioxidant defence in thyroid cancers. Cancer Lett; v.109; p. 231-235; 1996.
- ROBERTI; A. et al. Concomitance of hashimoto´s thyroiditis and differentiated thyroid cancer. Rev. Col. Bras. Cir. v. 33; n. 6; 2006.