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Epidemiological Data of Rotavirus, Norovirus and Sapovirus in Indonesia
Indonesia (CIMSA-ISMKI) - Universitas Airlangga, Surabaya
Viral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga Universitas Airlangga, Campus C, Jalan Dr. Ir. H. Soekarno, Mulyorejo, Surabaya, Indonesia
Prof. Sutjipto, dr., M.S., Ph.D
Prof. Sutjipto, dr., M.S., Ph.D
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Viral diarrhoea is caused by some viruses such as Rotavirus, Norovirus and Sapovirus. Rotavirus is the major cause of severe diarrhoea in children under 5 years old in developed and developing countries, including Indonesia. It is responsible for 20-70% of diarrhoea in children who were hospitalized, and 20% of all diarrhoea-related deaths in children under five years old around the world. In both developed and developing countries, especially in Indonesia, morbidity and mortality of its disease is high and usually it peaks in the dry season of July to August. Based on the epidemiology of this disease, viral diarrhoea project study is interested to find the virus strain and their epidemiology around Indonesia.
What is the aim of the project?
The aim of the project is to prove the epidemiological data and find the strain of Rotavirus, Norovirus and Sapovirus in Indonesia by analysing the molecular sample.
What techniques and methods are used?
1. Collecting stools from children under 17 years old as the sample from some hospitals in Surabaya, Sumatera and West Nusa Tenggara. 2. Making 10% stool suspension with centrifuges. 3. Making stool extraction. 4. For Norovirus and Sapovirus, we can directly do the Polymerase Chain Reaction (PCR). But for Rotavirus, we have to change it form into cDNA first. 5. Doing electrophoresis to see viruses’ genotype.
What is the role of the student?
- The student will mainly observe
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student could help in the lab and observe. But, the student mainly observing the research project. The student must attend the classes held by our professors about Clinical Microbiology, Safety and Introduction of viral diarrhoea. Then, the student will have a task to present some journals about the Rotavirus, Norovirus and Sapovirus from their own country. We also expected the student to have an ability in identifying stool specimens using PCR (Polymerase Chain Reactions), ELISA (Enzyme Linked Immunosorbent Assay). Finally, they must make a summary about their research activities in the past weeks.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, there is. The students will have some lectures about clinical microbiology, such as: 1. Basic Virology by Dr. Eko Budi Koendhori, dr., M.Kes, Sp.MK(K) 2. Viral Immunology by Dr. Agung Dwi Wahyu Widodo, dr., M.Si., Sp.MK 3. Basic PCR by Dr. Marijam, Apt., MS 4. Laboratory safety by dr. Lindawati Alimsardjono, MS, Sp.MK(K) 5. Introduction to Viral Diarrhoea by Dr. Juniastuti, dr., M.Kes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
There are no required skills needed prior the internship.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Koch; J. and Wiese-Posselt; M. (2011). Epidemiology of Rotavirus Infections in Children Less Than 5 Years of Age. The Pediatric Infectious Disease Journal; 30(2); pp.112-117
- Franck; K.; Fonager; J.; Ersbøll; A. and Böttiger; B. (2014). Norovirus Epidemiology in Community and Health Care Settings and Association with Patient Age; Denmark. Emerging Infectious Diseases; 20(7); pp.1123-1131.
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