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Elaborating potential Effect of Nutmeg Extract as Antisarcopenia Agent
Indonesia (CIMSA-ISMKI) - Universitas Padjajaran, Bandung
Physiology Division, Laboratory Animal and Physiology Molecular, Faculty of Medicine. Jalan Raya Bandung Sumedang km 21.6, Bandung.
Ronny Lesmana, MD, PhD
Ronny Lesmana, MD, PhD
Type of Research Project
- Basic science
What is the background of the project?
Sarcopenia is a chronic abnormality on aging people recognized by decreasing the nutrient status, the loss of mass muscle and its function whereby this will cause the decrease of life quality, and increase the risk number of morbidity and mortality towards elderly. Generally, Sarcopenia is identified by physical inactivity, mobility decrease, and slow movement, and low physical endurance. It is very essential to prevent the cause of losing mass and skeletal muscle function on aging patient in other to achieve proper therapy. From various herbals, nutmeg apparently has potential effect for anti-Sarcopenic.
What is the aim of the project?
To explore role of nutmeg extract as anti-Sarcopenic agent during aging.
What techniques and methods are used?
Twelve male wistar rats with age of 80 weeks were devided into 2 groups were treated nutmeg extract free safrole and myristicin for 10 weeks. Then, rats were sacrificed under anesthesia. Soleus and gastrocnemius muscles was collected, weighted, and stored at -80ºC until used. Ribose Nucleic Acid (RNA) was extracted from muscle sample using Trizol and expression of Myo D, Pax 7 and other genes were studied.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student need to develop the projects, arrange and handling animal in the lab, learn about RNA extraction, polymerase chain reaction (PCR) for gene expression and Western blot for protein levels. After experiment were done, student need to learn how to analyze data and make presentation regarding the project. Final target, the need to write a scientific report and submit the article to the journal
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, they need to read journal, learn method and we will give some exposure to the laboratory techniques.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Physiology; Previous experience of involvement in research project; Certificate of PBT TOEFL min. 550, and has active English conversation skill
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Thuong PT; Hung TM; Khoi NM; Nhung HTM; Chinh NT; Quy NT; et al. Cytotoxic and anti-tumor activities of lignans from the seeds of Vietnamese nutmeg Myristica fragrans. Archives of Pharmacal Research 2013;37:399–403. doi:10.1007/s12272-013-0185-4.
- Li F; Yang X-W; Krausz KW; Nichols RG; Xu W; Patterson AD; et al. Modulation of Colon Cancer by Nutmeg. Journal of Proteome Research 2015;14:1937–46. doi:10.1021/pr5013152.
- Yang X-N; Liu X-M; Fang J-H; Zhu X; et al. PPARα Mediates the Hepatoprotective Effects of Nutmeg. Journal of Proteome Research 2018;17:1887–97. doi:10.1021/acs.jproteome.7b00901
- Haijima A; Lesmana R; Shimokawa N; Amano I; Takatsuru Y; Koibuchi N. Differential neurotoxic effects of in utero and lactational exposure to hydroxylated polychlorinated biphenyl (OH-PCB 106) on spontaneous locomotor activity and motor coordination in young adult male mice. The Journal of Toxicological Sciences 2017;42:407–16. doi:10.2131/jts.42.407.
- Yau WW; Singh BK; Lesmana R; Zhou J; Sinha RA; et al. Thyroid hormone (T3) stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy. Autophagy 2018;15:131–50. doi:10.1080/15548627.2018.1511263.
- Zhou J; Sinha RA; Lesmana R; Yau WWY; Yen PM. Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro. Methods in Molecular Biology Thyroid Hormone Nuclear Receptor 2018:111–22. doi:10.1007/978-1-4939-7902-8_11.
- Other research by dr. Ronny Lesmana which can be found on PubMed: https://www.ncbi.nlm.nih.gov/ pubmed/?term=ronny%20lesmana
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