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Utilization of an in vitro erythroid culture system as a model to study erythropoiesis
Thailand (IFMSA-Thailand) - Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok
Asst. Prof. Vorapan Sirivatanauksorn, MD, PhD
Asst. Prof. Kongtana Trakarnsanga, MD, PhD
Type of Research Project
- Basic science
What is the background of the project?
Erythropoiesis is a continuous physiological process by which mature red blood cells are generated from multipotent stem cells. This complex process is regulated by several cytokines, growth factors and extracellular matrix proteins via intracellular signal transduction pathways. Over the past two decades,there have been a big progression in the development of in vitro erythroid culture systems that can on a small scale, generate RBCs from isolated stem cell populations. Erythroid cells differentiated from in vitro culture systems are similar to those in the bone marrow and can be used as a model to study erythropoiesis.
What is the aim of the project?
To use an in vitro erythroid culture system as a model to learn about human erythropoiesis.
What techniques and methods are used?
- Cell culture of erythroid cells - Hematological staining to study morphology of erythroid cells - Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis to separate protein lysates from erythroid cells - Western blot to detect protein expression in protein lysates from erythroid cells
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- Review about erythropoiesis and in vitro erythropoiesis - Haematopoietic stem cell isolation (observe) - Cell culture: to promote erythroid differentiation of haematopoietic stem cells - Hematological staining: to distinguish stages of cultured erythroid cells - Protein lysate preparation - Study of globin expression by Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis and western blot - An oral presentation at a departmental meeting
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
- Preliminary reading about erythropoiesis and in vitro erythropoiesis - Lecture about in vitro erythropoiesis - Biochemistry seminar (every Wednesday morning)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
1. Knowledge about human erythropoiesis 2. Cell culture and morphology identification skills 3. Protein sample preparation, protein identification and quantification skills
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- - Shah S; Huang X; Cheng L. Concise Review: Stem Cell-Based Approaches to Red Blood Cell Production for Transfusion. Stem Cells Translational Medicine. 2014;3(3):346-355. doi:10.5966/sctm.2013-0054. - Anstee DJ; Gampel A; Toye AM. Ex-vivo generation of human red cells for transfusion. Curr. Opin. Hematol. 2012;19:163–169.
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