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Testing the biocompatibility of biomaterials in regenerative medicine on in vitro and in vivo models
Serbia (IFMSA-Serbia) - University of Nis, Nis
Research Center for Biology and Human Genetics and Cellular and Tissue Engineering
Prof. dr Stevo Najman
Prof. dr Stevo Najman
Type of Research Project
- Basic science
What is the background of the project?
Biocompatibility testing of biomaterials is the basic level of testing materials used in regenerative medicine. The project deals with biomaterials used in regeneration and reparation of bone and surrounding connective tissue applicative in orthopedics, maxillofacial surgery, prosthetics, implantology. The materials are of the type of bone substituents based on hydroxyapatite and calcium phosphate, as well as collagen-based membranes. Studies are performed on cell cultures to determine the effect of biomaterials on cell viability and growth. Adipose derived stem cells used in the study of the effects on their differentiation into osteogenic cells. Implantation and grafting of the tested materials are carried out in in vivo models. Models of subcutaneous implantations into mice and bone defects of rat femur and calvary are used. After determined time points, the explantations and extraction of the material and its histological processing are carried out. Samples are HE stained, as well as specific for connective tissue and bone. The analysis of the regenerative and reparation process is performed morphometrically.
What is the aim of the project?
The aim of the project is to determine the biocompatibility of the tested biomaterials with cells and tissues through their interactions.
What techniques and methods are used?
Basic laboratory methods will be used: microscopy, cell culture, tissue processing and staining, tissue morphometry The state of the cell culture will be checkedby invert phase contrast microscopy.Subcultivation of some cultures will be followed by testing cell viability with tripan blue exclusion test. Furthermore, cell viability and proliferation will be tested using MTT and crystalline violet color after the cells are cultivated in different biomaterials with the purpose of determining biocompatibility between them. Morphometric analysis of cell cultures will be performed on inverted microscope.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students are expected to accept the way they work and behave in accordance with good laboratory practice. It is therefore necessary to wear adequate clothing and footwear. It is mandatory that the student complies with all safety requirements for work in the laboratory. An adequate cooperation with the supervisor and staff in laboratories and departments is expected.Any independent work will be performed according to the instructions and procedures in the laboratory. Prior to the independent work of the student, a check of his knowledge and skills will be made or he/she will perform the necessary training by the supervisor. The student will work with methods typical of the Cell Culture Laboratory. After prior acquaintance with the methods, he will independently check the state of the cell culture on the invert phase contrast microscope, replace the culture medium, passage the culture and subcultivation, centrifuge the cells, count the cells, check cell viability using tripan blue exclusinprincipl. For the purpose of testing biocompatibility of biomaterials, a student will assist in performing methods for testing cell viability and proliferation using MTT and crystalline violet color after cell cultivation in extracts of biomaterials. The student can independently work on the morphometric analysis of cell cultures on an inverted microscope. Tissue processing and staining, as wel as tissue morphometry the student will work under supervision. The student can assist in in vivo experiments on models of subcutaneous implantation of biomaterials to experimental animals (mice).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings in the area of Cell biology, Cell culture techniques, Histology, Biomaterials, Regenerative medicine, Tissue engineering will be provided for the student.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic laboratory work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Stevo J. Najman; Vladimir J. Cvetković; Jelena G. Najdanović; SanjaStojanović; Marija Đ. Vukelić- Nikolić; Ivica Vučković; Dragan Petrović. Ectopic osteogenic capacity of freshly isolated adipose- derived stromal vascular fraction cells supported with platelet-rich plasma: a simulation of intraoperative procedure. Journal of Cranio-Maxillofacial Surgery 2016; 44(10):1750–1760.
- Vuković Jovana; Babić Marija; Antić Katarina; FilipovićJovanka; StojanovićSanja; NajmanStevo; TomićSimonida. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. Materials Chemistry and Physics 2016; 175: 158–163.
- Barbeck Mike; NajmanStevo; StojanovićSanja; MitićŽarko; ŽivkovićJelena; Choukroun Joseph; KovačevićPredrag; Sader Robert; Kirkpatrick C James; GhanaatiShahram. Addition of blood to a phycogenic bone substitute leads to increasedin vivo vascularization. Biomedical Materials 2015; 10(5). DOI:10.1088/1748-6041/10/5/055007
- Cvetković Vladimir J.; NajdanovićJelena G.; Vukelić- NikolićMarija Đ.; StojanovićSanja; NajmanStevo J. Osteogenic potential of in vitro osteo-induced adipose-derived mesenchymal stem cells combined with platelet-rich plasma in an ectopic model. International Orthopaedics. 2015; 39(11): 2173- 2180.
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