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Toxicological Evaluation of Dithiocarbamate Fungicide and Its Bio-metabolite on the Kidney and Renal-derived Cell Lines
Korea (KMSA) - Catholic Kwandong university, Gangwon province
Department of Physiology, College of Medicine, Catholic Kwandong University, 24, 579 Bumil, Gangneung, Gangwon, Republic of Korea, 25601
Seong-Chun Kwon, Ph.D.
Prof. Byong-Gon PARK, Ph.D.
Type of Research Project
- Basic science
What is the background of the project?
The Endocrine Society released a statement on endocrine-disrupting chemicals (EDCs), specifically listing female and male reproductive systems, hormone-sensitive cancers in females, prostate cancer in males, the thyroid, and neurodevelopment and neuroendocrine systems as being affected by exposure to EDCs. Endocrine disruptors are chemicals that can interfere with endocrine systems at specific amounts and can eventually cause cancerous tumors, birth defects, and other developmental disorders. Short- and long-term exposures to mancozeb, a polymeric complex zinc and manganese salts of ethylene bisthiocarbamate, and its bio-metabolite are associated with neurotoxic, developmental disability, immunotoxic, and carcinogenic effects in humans and experimental animals.
What is the aim of the project?
Toxicological evaluation of dithiocarbamate fungicide mancozeb and ethylenethiourea, bio-metabolite of mancozeb, on the kidney of the C57BL/6 mouse in vivo, and podocytes and renal proximal tubular epithelial cell lines (HK-2) in vitro.
What techniques and methods are used?
1) Paraffin-embedded tissue preparation & staining 2) Affymetrix miRNA(Ribonucleic acid) chip and DNA(Deoxyribonucleic acid) chip analysis 3) Reverse transcription polymerase chain reaction(RT-PCR), quantitative PCR(qPCR), & western blotting 4) Fluorescent dye-based Ca2+ imaging in VSMCs(Vascular smooth muscle cells) in automated Flexstation 3
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Student takes part in project as a research assistant. Student will be experiment for paraffin-embedded tissue block and staining, miRNA and DNA chip analysis, q-PCR (Polymerase Chain Reaction), fluorescent dye-based in vitro assay, and long-term exposure animal model study. Student will read about project-related journal articles. Student will be supervised with handling the task. Department wish student acquire knowledge and techniques for basic medical science.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Department provide a preliminary reading & lecture for research project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Minor requirement for gene and protein analysis, and bioinformatics.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Runkle J; Flocks J; Economos J; Dunlop AL. A systemic review of mancozeb as a reproductive and developmental hazard. Environ Int; 99; 29-42; 2017.
- Phua YL and Ho J. MicroRNAs in the pathogenesis of cystic kidney disease. Curr Opin Pedistr; 27(2); 219-226; 2015
- Kwon D; Chung HK; Shin WS; Park YS; Kwon SC; Song JS; Park BG. Toxicological evaluation of dithiocarbamate fungicide mancozeb on the endocrine functions in male rats. Mol Cell Toxicol; 14(1); 2018
- Chung HK; Cho WC; Park HY; Choi SH; Kwon D; Shin WS; Song JS; Park BG. Chronic exposure to ethylenethiourea induces kidney injury and polycystic kidney in mice. Mol Cell Toxicol (In Press)
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