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Detection of antimicrobial resistance in the Lebanese community and hospital setting
University of Balamand
MICROB-Saint George hospital, Achrafieh, Beirut, Lebanon
Dr. Eid Azar
Dr. Eid Azar
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The emergence and spread of multi drug resistant organisms (MDRO), for example Enterobacteriacea, are nowadays considered as one of the major public health concern worldwide and especially in Lebanon. These organisms can cause severe infections with high morbidity, mortality, and health care costs. Previous studies report varying rates of MDRO carriage in the hospital setting and the community as well. Due to the limited antibacterial options, and the lack of production of new antibiotics, the treatment of infections caused by these organisms became challenging.
What is the aim of the project?
The aim of the project is to detect the prevalence and the anti-microbial profiles of MDROs throughout hospitals and communities in Lebanon.
What techniques and methods are used?
Basic techniques are used such as: 1. Collection of samples (collection of swabs from participants) 2. Isolation of isolates (four quadrant streaking technique on regular and antibiotic containing agars/ broth followed by incubation of at least 24 hours) 3. Detecting the types of bacteria (gram staining, gallery (ex. API 20E)) 4. Identifying the isolates’ antimicrobial profile (mainly using the Kirby-Bauer diffusion method and in some special cases PCR may be used) Tools: loops, petri dishes, bensen burner, slides, forceps.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to first of all be on time in order to collect the specimen. The technique used for sample collection will depend on the type of specimen and the bacterial species. The student should be able to perform the techniques used during the study namely bacterial culture (on solid agar or broth), gram staining (basic procedure), preservation of the isolates (using glycerol), perform the antimicrobial susceptibility profile using the assigned antibiotic disks and finally report the results properly by referring the CLSI guidelines.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic microbiological knowledge
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Mouawad; R.; Afif; C.; Azar; E.; Dahdouh; E.; Masri; K.; Irani; J.; & Daoud; Z. (2013). Effect of antibiotic consumption on resistance of Pseudomonas aeruginosa isolated from Lebanese patients with emphasis on MBL production. Advances in Microbiology; 3(04); 382.
- Jallad; M. A.; Naoufal; R.; Irani; J.; & Azar; E. (2015). Extended spectrum beta-lactamase carriage state among elderly nursing home residents in Beirut. The Scientific World Journal; 2015.
- Ballouz; T.; Aridi; J.; Afif; C.; Irani; J.; Lakis; C.; Nasreddine; R.; & Azar; E. (2017). Risk factors; clinical presentation; and outcome of acinetobacter baumannii bacteremia. Frontiers in cellular and infection microbiology; 7; 156.
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