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Study of the extracellular matrix in developing kidney
Serbia (IFMSA-Serbia) - University of Nis, Nis
Department for Histology and Embryology
Goran Radenković, MD, PhD, associate professor
Vladimir Petrović, MD, PhD, assistant professor
Type of Research Project
- Basic science
What is the background of the project?
We have recently started working on a project on extracellular matrix in fetal kidneys. It is important to know the normal developmental of human kidney for better understanding of various congenital renal conditions. Kidney samples for this project are obtained at autopsies from infants that died in utero or within 24 h after birth. Using histochemical and immunohistochemical procedures the following components of the extracellular matrix (ECM) are being examined: collagen type I, collagen type III, collagen type IV, laminin and fibronectin. The quantification of the examined ECM components will be done by determining their volume density in the whole kidney, as well as in specific structural parts of developing kidney. The obtained results will be statistically compared and the variations in the aforementioned components will be noted.
What is the aim of the project?
The aim of the project is to describe the topographic distribution of the different components of the fetal kidney extracellular matrix, to quantify them using morphometrical analyses and to establish the pattern of fetal kidney ECM development.
What techniques and methods are used?
The material used for the research will consist of embryonic and fetal kidneys in all three trimesters of development. Techniques and methods that are used are : tissue processing (slicing, preparing fetal kidney tissue for staining), staining : we are working with hematoxyline-eosine staining, histochemical stainings (Van Gison staining, staining of reticular fibers), immunohystochemical staining. Morphometric analysis using Image J software, statistical analysis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will master the techniques of tissue processing, as well as the different histochemical staining procedures (hematoxyline-eosine staining, Van Gison staining, staining od reticular fibers). Also, the student will be able to learn the technique of immunohistochemical staining and will be able to perform the procedure on his own. The project also includes the morphometric analysis of the foetal kidney tissue, so the student will be aquainted with the morphometrical procedures performed using the software Image J.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, the student will learn laboratory procedures and morphometric analysis principles during the joint work with the tutor. All the explanations will be provided during the practical work.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
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