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Sanger sequencing and next-generation sequencing are the main techniques used in this project. Variations in BKPyV genome will be investigate using Next-generation sequencing, allowing identification of subpopulation as low as 1% of the total viral population.
For CMPK1 genotyping, Sanger sequencing will be used to assess the presence of amino acid changes in the enzyme responsible for the activation of cidofovir.