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Understanding the role of post-translational protein modifications in the regulation of the catalytic activity of enzymes
Korea (KMSA) - Chungnam university, Daejeon
Department of Biochemistry and Department of Medical Science
Type of Research Project
- Basic science
What is the background of the project?
Post-translational protein modifications (PTMs) refer to covalent addition or removal of functional groups or proteins to proteins after protein synthesis, which increases the diversity of proteomes. For example, proteins not only can be attached by small molecules such as methyl, acetyl groups or phosphate but also can be covalently modified by proteins. Notably, PTMs have emerged as critical regulatory processes, such as in the control of cell cycle, stress response, signaling transduction, and immune response.
What is the aim of the project?
1. Identifying Post-translational protein modifications (PTMs) that modulate enzymes. 2. Elucidating the role of PTMs in the regulation of the activity of enzymes 3. Elucidating the crosstalk of PTMs in the regulation of the activity of enzymes
What techniques and methods are used?
1. Immunoprecipitation to determine protein-protein interactions. 2. Immunoblotting to capture the proteins of interests. 3. Ni2+-Nitrilotriacetic acid (NTA) pull-down analysis to purify the proteins of interests. 4. Immunocytochemistry to determine the subcellular localization of proteins. 5. Sodium lauryl sulfate (SDS) polyacrylamide gel electrophoresis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (minimum 10 lines - please, provide detailed explanation) By the end of the exchange, student should have the ability to design simple experiment (regarding to this research). 4 weeks are short period to fully understand this research. So before exchange, we recommend the student to read given articles (which is mentioned below). We may ask some questions about this research before starting exchange. By the end of the exchange, we expect the student to fluently communicate with laboratory colleagues. The student may understand and get familiar with basic experimental procedures (i.e. Western blotting, Protein purifying). Of course, English speaking / listening skill is mandatory to accomplish this. Finally, the student is expected to develop ability to interpret given data. So before exchange, We recommend the student to read some other papers which are related to this subject.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1. The student can attend our lab seminar (Journal Club) every week. 2. Preliminary readings and introductory lectures will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
What skills are required of the student? Skills to read and understand research articles
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Kwon YT and Ciechanover A . The Ubiquitin Code in the Ubiquitin-Proteasome System and Autophagy Trends Biochem Sci. 2017 Nov;42(11):873-886
- Ji CH and Kwon YT . Crosstalk and Interplay between the Ubiquitin-Proteasome System and Autophagy. Mol Cells. 2017 Jul 31;40(7):441-449
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