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Bacteriological monitoring among medical students for the carriage of pathogenic staphylococcus to determine antibacterial sensitivity
Kazakhstan (KazMSA) - Astana Medical University, AMU, Astana, Kazakhstan
Department of microbiology and virology named by Sarbasova Sh. I.
d.m.s., Professor – Dusmagambetov Marat Uteuovich
Botbayeva Madina Turlybekovna: +77019877332
Required: English Accepted: English, Kazakh, Russian.
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Throughout the world for many decades the etiological role of gram-positive cocci in the development of inflammatory diseases doesn't lose its relevance. The proportion of inflammatory diseases account for 30-35% of all surgical diseases, of which from 25 to 50% are etiologically caused by cocci. The greatest danger is the medical staff as a carrier of strains of staphylococcus. Early detection of staphylococcal carriers among future medical workers gives the opportunity to receive timely professional advice and, if necessary, rehabilitation of carriers before students come to work practice or work in a medical institution. All the tasks of this research work will help prevent outbreaks of nosocomial infection of staphylococcal etiology in medical institutions, and thus preserve the health of patients, therefore, the main principle of modern medicine - disease prevention will be implemented.
What is the aim of the project?
The aim of the research is to determine the route of transmission of staphylococcal infections; isolation and identification of pathogenic staphylococcus; study of the clinical manifestations of staphylococcal infections; determination of antibacterial sensitivity.
What techniques and methods are used?
1. Questioning students to assess the condition of students. Questions: general information, anthropometric data, the presence of chronic diseases, immune status. 2. The selection of bacteria from the original sample. Take a sterile tube with a swab between IV and V fingers and a spatula with three other fingers in your left hand. Ask the patient to open his mouth wide, fix the tongue, pushing the tongue down with the spatula. We introduce a tampon into the oral cavity, without touching the mucous membranes of the necks, teeth, tongue, soft palate. Swab the mucus from the palatine arches of the tonsils. 3. Sowing bacteria and growing a pure culture (sowing on a Petri dish), studying its properties; 4. Bacteriological study of the material (macro and micro analysis - determination of the morphological, biochemical, toxigenic and antigenic properties of a bacterial culture); 5. Determination of antibiotic sensitivity through paper disks based on the diffusion of the antibiotic into the culture medium. 6. Evaluation of the obtained research results is carried out using a summary and grouping materials of statistical research and is presented in the form of various graphical tables.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student conducts a survey among students on their own (in preferred language) and participates in the collection of primary tests under the supervision of a tutor. The student can carry out seeding bacteriological colonies, with strict observance of safety measures (wearing masks and gloves during sampling and use of disinfectants). The determination of the morphological, biochemical, toxigenic and antigenic properties of a bacteria culture, as well as the work with the microscope, is carried out by a student under the supervision of a tutor. The student makes conclusions based on biostatistical analyzes presented in the form of graphical tables on paper or on MS Excel, SPSS programs.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will receive theoretical training in the form of handouts about microorganisms, methods of their seeding and differentiation. The supervisor will explain the introductory material and answer additional questions.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Next skills are required: the skills for procedure with scientific literature, microscope, laboratory equipment. Students should have knowledge of microbiology, biostatistical methods of analysis. Subjects passed: Microbiology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- “An introduction to staphylococcus aureus; and techniques for identifying and quantifying s. Aureus adhesins in relation to adhesion to biomaterials”; L.G. Harris; S.J. Foster; and R.G. Richards; European Cells and Materials Vol. 4. 2002 (pages 39-60)
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