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Evaluation the therapeutic role of histone deacetylase inhibitors in chemoresistant bladder cancer via intravesical instillation
Taiwan (FMS-Taiwan) - Chung Shan Medical University, Taichung
Type of Research Project
- Basic science
What is the background of the project?
Bladder cancer is common malignancy worldwide. Owing to the high recurrence rate of bladder cancer patients after tumor resection, intravesical instillation of chemotherapy or Bacillus Calmette-Guerin (BCG) is recommend for prevention. However, BCG is out of stock for years and study for alternative treatment is necessary. During the tumorigenesis process of bladder cancer, mutations and copy number variations of histone modification associated genes are common. Among them, histone deacetylases (HDACs) play the key role in cell cycle regulation, cell apoptosis inhibition, and promotion of tumor malignancy. This suggests the potential therapeutic role of histone deacetylase inhibitors (HDAC inhibitors) in bladder cancer.
What is the aim of the project?
We will investigate the role and therapeutic mechanisms of HDAC inhibitors in Mitomycin C and Doxorubicin resistant cells; we will use the primary bladder cancer cell lines to evidence the role of HDAC inhibitors. Furthermore, we will proof our findings with animal model with intravesical instillation treatment.
What techniques and methods are used?
MTT assay; bladder cancer animal model, Western blot; flow cytometry; immunohistochemistry staining MMT assay: (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) ;The MTT assay is a colorimetric assay for assessing cell metabolic activity.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The methods above are what we expect the student may be equipped with. The degree of involvement of the students in the practical work depends on students’ skills. If skills are performed fluently or already had quite enough experience, he/she may participate in more of our practical experiment. The tutor will decide the degree of involvement after students’ practical work. Thus, we hope that students can read skills that are required first and decide they would like to participate in this project or not later. Assignments are given according to personal ability.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
MTT assay; bladder cancer animal model, Western blot; flow cytometry; immunohistochemistry staining We hope that students can learn and know those skills before the exchange. If they don’t, they can ask and learn during the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Sci Rep. 2016 Dec 20;6:39257. doi: 10.1038/srep39257. Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis. Haldar S1; Dru C2; Mishra R1; Tripathi M1; Duong F1;3; Angara B1;3; Fernandez A1;3; Arditi M4; Bhowmick NA1;3.
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